UniProt Accession

 RALA_RAT; P63322; P05810 

Genbank Protein ID

 L19698 

Genbank Nucleotide ID

 AAA42003.1 

Protein Name

 Ras-related protein Ral-A 

Protein Synonyms/Alias

  

Gene Name

 Rala 

Gene Synonyms/Alias

 Ral; Ral-a 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
134	NKSDLEDKRQVSVEE	acetylation	[1]
143	QVSVEEAKNRADQWN	ubiquitination	[2]
159	NYVETSAKTRANVDK	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity). 

Sequence Annotation

 NP_BIND 21 28 GTP (By similarity).
 NP_BIND 68 72 GTP (By similarity).
 NP_BIND 127 130 GTP (By similarity).
 MOTIF 43 51 Effector region (By similarity).
 MOD_RES 203 203 Cysteine methyl ester (By similarity).
 LIPID 203 203 S-geranylgeranyl cysteine (By  

Keyword

 Cell cycle; Cell division; Cell membrane; Complete proteome; Exocytosis; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 206 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
 GO:0032154; C:cleavage furrow; ISS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030139; C:endocytic vesicle; IEA:Compara.
 GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0031755; F:Edg-2 lysophosphatidic acid receptor binding; ISS:UniProtKB.
 GO:0005525; F:GTP binding; NAS:RGD.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0031532; P:actin cytoskeleton reorganization; IEA:Compara.
 GO:0000910; P:cytokinesis; ISS:UniProtKB.
 GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
 GO:0051665; P:membrane raft localization; ISS:UniProtKB.
 GO:0051491; P:positive regulation of filopodium assembly; IEA:Compara.
 GO:0017157; P:regulation of exocytosis; ISS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR020849; Small_GTPase_Ras. 

Pfam

 PF00071; Ras 

SMART

 SM00173; RAS 

PROSITE

 PS51421; RAS 

PRINTS

 PR00449; RASTRNSFRMNG.