CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006103
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neutral trehalase 
Protein Synonyms/Alias
 Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase 
Gene Name
 NTH1 
Gene Synonyms/Alias
 NTH; YDR001C; YD8119.07C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
163LQELTIAKSFGRHQIubiquitination[1]
221KIDTPGAKNPRIYVPubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301
Functional Description
  
Sequence Annotation
 REGION 309 310 Substrate binding (By similarity).
 REGION 355 357 Substrate binding (By similarity).
 ACT_SITE 478 478 Proton donor/acceptor (By similarity).
 ACT_SITE 674 674 Proton donor/acceptor (By similarity).
 BINDING 302 302 Substrate (By similarity).
 BINDING 346 346 Substrate (By similarity).
 BINDING 355 355 Substrate (By similarity).
 BINDING 476 476 Substrate; via carbonyl oxygen (By
 MOD_RES 20 20 Phosphoserine.
 MOD_RES 21 21 Phosphoserine.
 MOD_RES 23 23 Phosphoserine.
 MOD_RES 58 58 Phosphothreonine.
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 66 66 Phosphoserine.
 MOD_RES 83 83 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Glycosidase; Hydrolase; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 751 AA 
Protein Sequence
MSQVNTSQGP VAQGRQRRLS SLSEFNDPFS NAEVYYGPPT DPRKQKQAKP AKINRTRTMS 60
VFDNVSPFKK TGFGKLQQTR RGSEDDTYSS SQGNRRFFIE DVDKTLNELL AAEDTDKNYQ 120
ITIEDTGPKV LKVGTANSYG YKHINIRGTY MLSNLLQELT IAKSFGRHQI FLDEARINEN 180
PVNRLSRLIN TQFWNSLTRR VDLNNVGEIA KDTKIDTPGA KNPRIYVPYD CPEQYEFYVQ 240
ASQMHPSLKL EVEYLPKKIT AEYVKSVNDT PGLLALAMEE HFNPSTGEKT LIGYPYAVPG 300
GRFNELYGWD SYMMALGLLE ANKTDVARGM VEHFIFEINH YGKILNANRS YYLCRSQPPF 360
LTEMALVVFK KLGGRSNPDA VDLLKRAFQA SIKEYKTVWT ASPRLDPETG LSRYHPNGLG 420
IPPETESDHF DTVLLPYASK HGVTLDEFKQ LYNDGKIKEP KLDEFFLHDR GVRESGHDTT 480
YRFEGVCAYL ATIDLNSLLY KYEIDIADFI KEFCDDKYED PLDHSITTSA MWKEMAKIRQ 540
EKITKYMWDD ESGFFFDYNT KIKHRTSYES ATTFWALWAG LATKEQAQKM VEKALPKLEM 600
LGGLAACTER SRGPISISRP IRQWDYPFGW APHQILAWEG LRSYGYLTVT NRLAYRWLFM 660
MTKAFVDYNG IVVEKYDVTR GTDPHRVEAE YGNQGADFKG AATEGFGWVN ASYILGLKYM 720
NSHARRALGA CIPPISFFSS LRPQERNLYG L 751 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0004555; F:alpha,alpha-trehalase activity; TAS:SGD.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005993; P:trehalose catabolic process; IMP:SGD. 
Interpro
 IPR008928; 6-hairpin_glycosidase-like.
 IPR001661; Glyco_hydro_37.
 IPR018232; Glyco_hydro_37_CS.
 IPR011120; Trehalase_Ca-bd. 
Pfam
 PF01204; Trehalase
 PF07492; Trehalase_Ca-bi 
SMART
  
PROSITE
 PS00927; TREHALASE_1
 PS00928; TREHALASE_2 
PRINTS
 PR00744; GLHYDRLASE37.