CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000876
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin-linked protein kinase 
Protein Synonyms/Alias
  
Gene Name
 Ilk 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
85GHRDIVQKLLQYKADubiquitination[1]
139YGEMPVDKAKAPLREubiquitination[1]
154LLRERAEKMGQNLNRubiquitination[1]
165NLNRIPYKDTFWKGTubiquitination[1]
170PYKDTFWKGTTRTRPubiquitination[1]
191KHSGIDFKQLNFLAKubiquitination[1]
209NHSGELWKGRWQGNDubiquitination[1]
426PHVCKLMKICMNEDPacetylation[2, 3, 4, 5]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (By similarity). 
Sequence Annotation
 REPEAT 2 30 ANK 1.
 REPEAT 31 63 ANK 2.
 REPEAT 64 96 ANK 3.
 REPEAT 97 129 ANK 4.
 REPEAT 130 174 ANK 5.
 DOMAIN 193 446 Protein kinase.
 NP_BIND 199 207 ATP (By similarity).
 REGION 33 139 Interaction with LIMS1 (By similarity).
 REGION 180 212 PH-like; mediates interaction with
 BINDING 220 220 ATP (By similarity).
 MOD_RES 186 186 Phosphoserine.  
Keyword
 ANK repeat; ATP-binding; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI 60
NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV 120
ANGALVSICN KYGEMPVDKA KAPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG 180
TLNKHSGIDF KQLNFLAKLN ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP 240
RLRIFSHPNV LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD 300
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA 360
LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH 420
VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK 452 
Gene Ontology
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0043034; C:costamere; IEA:Compara.
 GO:0043198; C:dendritic shaft; IEA:Compara.
 GO:0005925; C:focal adhesion; IDA:MGI.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0030017; C:sarcomere; IEA:UniProtKB-SubCell.
 GO:0043195; C:terminal bouton; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; ISO:MGI.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0001658; P:branching involved in ureteric bud morphogenesis; IDA:MGI.
 GO:0007569; P:cell aging; IEA:Compara.
 GO:0007050; P:cell cycle arrest; IEA:Compara.
 GO:0030030; P:cell projection organization; IMP:MGI.
 GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; IMP:MGI.
 GO:0043206; P:extracellular fibril organization; IEA:Compara.
 GO:0007229; P:integrin-mediated signaling pathway; ISO:MGI.
 GO:0032288; P:myelin assembly; IEA:Compara.
 GO:0022011; P:myelination in peripheral nervous system; IMP:MGI.
 GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0006469; P:negative regulation of protein kinase activity; IEA:Compara.
 GO:0014912; P:negative regulation of smooth muscle cell migration; IEA:Compara.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0021675; P:nerve development; IMP:MGI.
 GO:0048812; P:neuron projection morphogenesis; IEA:Compara.
 GO:0018105; P:peptidyl-serine phosphorylation; IMP:MGI.
 GO:0045773; P:positive regulation of axon extension; IEA:Compara.
 GO:0030513; P:positive regulation of BMP signaling pathway; IMP:BHF-UCL.
 GO:0090263; P:positive regulation of canonical Wnt receptor signaling pathway; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IMP:MGI.
 GO:0001954; P:positive regulation of cell-matrix adhesion; IEA:Compara.
 GO:0050775; P:positive regulation of dendrite morphogenesis; IEA:Compara.
 GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
 GO:0045663; P:positive regulation of myoblast differentiation; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IMP:BHF-UCL.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0043491; P:protein kinase B signaling cascade; IMP:MGI.
 GO:0032956; P:regulation of actin cytoskeleton organization; IEA:Compara.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IEA:Compara. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR016253; Integrin-linked_kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom. 
Pfam
 PF00023; Ank
 PF07714; Pkinase_Tyr 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS