CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010173
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase 
Protein Synonyms/Alias
  
Gene Name
 ubiF 
Gene Synonyms/Alias
 yleB; b0662; JW0659 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
151DLQELELKGGEVIRAacetylation[1]
159GGEVIRAKLVIGADGacetylation[1]
251QLQAEIAKHFPSRLGacetylation[1]
332WASYPVLKRYQMRRMacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol. 
Sequence Annotation
  
Keyword
 Complete proteome; FAD; Flavoprotein; Monooxygenase; Oxidoreductase; Reference proteome; Ubiquinone biosynthesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 391 AA 
Protein Sequence
MTNQPTEIAI VGGGMVGGAL ALGLAQHGFA VTVIEHAEPA PFVADSQPDV RISAISAASV 60
SLLKGLGVWD AVQAMRCHPY RRLETWEWET AHVVFDAAEL KLPLLGYMVE NTVLQQALWQ 120
ALEAHPKVTL RVPGSLIALH RHDDLQELEL KGGEVIRAKL VIGADGANSQ VRQMAGIGVH 180
AWQYAQSCML ISVQCENDPG DSTWQQFTPD GPRAFLPLFD NWASLVWYDS PARIRQLQNM 240
NMAQLQAEIA KHFPSRLGYV TPLAAGAFPL TRRHALQYVQ PGLALVGDAA HTIHPLAGQG 300
VNLGYRDVDA LIDVLVNARS YGEAWASYPV LKRYQMRRMA DNFIMQSGMD LFYAGFSNNL 360
PPLRFMRNLG LMAAERAGVL KRQALKYALG L 391 
Gene Ontology
 GO:0008682; F:2-octoprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase activity; IMP:EcoliWiki.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; ISS:EcoCyc.
 GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IEA:InterPro.
 GO:0006744; P:ubiquinone biosynthetic process; IMP:EcoCyc. 
Interpro
 IPR002938; mOase_FAD-bd.
 IPR003042; Rng_hydrolase-like.
 IPR018168; Ubi_Hdrlase_CS.
 IPR010971; Ubi_Hdrxlases. 
Pfam
 PF01494; FAD_binding_3 
SMART
  
PROSITE
 PS01304; UBIH 
PRINTS
 PR00420; RNGMNOXGNASE.