UniProt Accession

 PCID2_HUMAN; Q5JVF3; A6NK09; Q3ZCX1; Q5TC57; Q5TC58; Q9H7K1; Q9HBZ7; Q9NUK6; Q9NVY1; Q9NW44; Q9NWH3 

Genbank Protein ID

 AK000888; AK001185; AK001302; AK002167; AK023313; AK024478; AF183426; AL137002; AL136221; AL137002; AL137002; AL136221; AL136221; AL137002; AL137002; AL136221; AL136221; AL137002; AL137002; AL136221; BC016614; BC031246 

Genbank Nucleotide ID

 BAA91407.1; BAA91542.1; BAA91611.1; BAA92118.1; BAB14521.1; BAB15768.1; AAG09695.1; CAI41391.1; CAI13794.1; CAI13794.1; CAI41378.1; CAI41378.1; CAI13793.1; CAI13793.1; CAI41377.1; CAI41377.1; CAI13792.1; CAI13792.1; CAI41379.1; CAI41379.1; AAH16614.1; AAH31246.1 

Protein Name

 PCI domain-containing protein 2 

Protein Synonyms/Alias

 CSN12-like protein 

Gene Name

 PCID2 

Gene Synonyms/Alias

 HT004 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
32	CAELVSFKHPHVANP	ubiquitination	[1, 2]
49	QMASPEEKCQQVLEP	ubiquitination	[1]
128	NADQQLVKKGKSKVG	ubiquitination	[1, 3, 4, 5]
133	LVKKGKSKVGDMLEK	acetylation	[2, 6, 7, 8]
133	LVKKGKSKVGDMLEK	ubiquitination	[1]
201	AIDSSNLKDDYSTAQ	ubiquitination	[1, 2, 3, 4, 5, 9, 10]
213	TAQRVTYKYYVGRKA	ubiquitination	[1, 2, 4, 5, 9, 10, 11]
219	YKYYVGRKAMFDSDF	ubiquitination	[1, 2]
227	AMFDSDFKQAEEYLS	ubiquitination	[10]
383	YISHQHQKLVVSKQN	acetylation	[2]
388	HQKLVVSKQNPFPPL	ubiquitination	[1, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [7] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation (By similarity). 

Sequence Annotation

 DOMAIN 277 388 PCI.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 399 AA 

Protein Sequence

Gene Ontology

 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:2000117; P:negative regulation of cysteine-type endopeptidase activity; IMP:UniProtKB.
 GO:0045579; P:positive regulation of B cell differentiation; IEA:Compara.
 GO:0090267; P:positive regulation of mitotic cell cycle spindle assembly checkpoint; IMP:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0043488; P:regulation of mRNA stability; IMP:UniProtKB.
 GO:0048536; P:spleen development; ISS:UniProtKB. 

Interpro

 IPR013143; PAM.
 IPR000717; PCI_dom. 

Pfam

 PF01399; PCI 

SMART

 SM00753; PAM 

PROSITE

  

PRINTS