UniProt Accession

 SNRPA_HUMAN; P09012 

Genbank Protein ID

 M60784; M60779; M60780; M60781; M60782; M60783; X06347; BC000405; BC008290 

Genbank Nucleotide ID

 AAA61245.1; AAA61245.1; AAA61245.1; AAA61245.1; AAA61245.1; AAA61245.1; CAA29653.1; AAH00405.1; AAH08290.1 

Protein Name

 U1 small nuclear ribonucleoprotein A 

Protein Synonyms/Alias

 U1 snRNP A; U1-A; U1A 

Gene Name

 SNRPA 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
20	YINNLNEKIKKDELK	ubiquitination	[1, 2]
60	GQAFVIFKEVSSATN	acetylation	[3]
60	GQAFVIFKEVSSATN	ubiquitination	[1, 2]
88	PMRIQYAKTDSDIIA	ubiquitination	[1, 2, 4]
96	TDSDIIAKMKGTFVE	ubiquitination	[1, 2]
98	SDIIAKMKGTFVERD	ubiquitination	[1, 2]
268	RDALQGFKITQNNAM	ubiquitination	[1, 2]
276	ITQNNAMKISFAKK*	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro. 

Sequence Annotation

 DOMAIN 10 89 RRM 1.
 DOMAIN 208 282 RRM 2.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 60 60 N6-acetyllysine.
 MOD_RES 131 131 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 282 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005681; C:spliceosomal complex; IDA:HGNC.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IDA:UniProtKB.
 GO:0017069; F:snRNA binding; IEA:InterPro.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:HGNC. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR024888; U1_snRNP_A/U2_snRNP_B''. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS