UniProt Accession

 PICAL_MOUSE; Q7M6Y3; Q3TS04; Q811P1; Q8BUF6; Q8CIH8; Q8R0A9; Q8R3E1; Q8VDN5; Q921L0 

Genbank Protein ID

 AY206701; BK001028; BC011470; BC021491; BC023843; BC025566; BC027116; BC057683; AK085472; AK162360 

Genbank Nucleotide ID

 AAO17153.1; DAA01470.1; AAH11470.1; AAH21491.1; AAH23843.1; AAH25566.1; AAH27116.1; AAH57683.1; BAC39454.2; BAE36872.1 

Protein Name

 Phosphatidylinositol-binding clathrin assembly protein 

Protein Synonyms/Alias

 Clathrin assembly lymphoid myeloid leukemia; CALM 

Gene Name

 Picalm 

Gene Synonyms/Alias

 Calm; Fit1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
460	DSGGGLLKPTVASQN	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Plays a crucial role in fetal and adult hematopoiesis, and normal prenatal and postnatal growth and viability. 

Sequence Annotation

 DOMAIN 14 145 ENTH.
 REGION 221 294 Interaction with FAM64A (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 443 443 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Coated pit; Complete proteome; Cytoplasmic vesicle; Developmental protein; Endocytosis; Golgi apparatus; Membrane; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 660 AA 

Protein Sequence

Gene Ontology

 GO:0030122; C:AP-2 adaptor complex; IEA:Compara.
 GO:0005905; C:coated pit; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005622; C:intracellular; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005545; F:1-phosphatidylinositol binding; ISS:UniProtKB.
 GO:0030276; F:clathrin binding; ISS:UniProtKB.
 GO:0007409; P:axonogenesis; IMP:BHF-UCL.
 GO:0048268; P:clathrin coat assembly; IEA:InterPro.
 GO:0048813; P:dendrite morphogenesis; IMP:BHF-UCL.
 GO:0016197; P:endosomal transport; IEA:Compara.
 GO:0030097; P:hemopoiesis; IMP:UniProtKB.
 GO:0048261; P:negative regulation of receptor-mediated endocytosis; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0031623; P:receptor internalization; IEA:Compara.
 GO:0006898; P:receptor-mediated endocytosis; IDA:UniProtKB.
 GO:0032880; P:regulation of protein localization; IEA:Compara. 

Interpro

 IPR011417; ANTH_dom.
 IPR014712; Clathrin_Pinositid-bd_GAT-like.
 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N. 

Pfam

 PF07651; ANTH 

SMART

 SM00273; ENTH 

PROSITE

 PS50942; ENTH 

PRINTS