UniProt Accession

 UB2J2_HUMAN; Q8N2K1; A8MYC7; Q504T9; Q96N26; Q96T84 

Genbank Protein ID

 AF296658; AK056065; AK075017; AK291935; AL162741; CH471183; CH471183; BC094777; BC104890; BC113430; BC143657; BM544827 

Genbank Nucleotide ID

 AAK52609.1; BAB71086.1; BAC11355.1; BAF84624.1; CAI23257.1; EAW56256.1; EAW56259.1; AAH94777.1; AAI04891.1; AAI13431.1; AAI43658.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 J2 

Protein Synonyms/Alias

 Non-canonical ubiquitin-conjugating enzyme 2; NCUBE-2 

Gene Name

 UBE2J2 

Gene Synonyms/Alias

 NCUBE2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
18	TTATQRLKQDYLRIK	ubiquitination	[1]
25	KQDYLRIKKDPVPYI	ubiquitination	[1]
26	QDYLRIKKDPVPYIC	ubiquitination	[1]
64	EGGYYHGKLIFPREF	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) (By similarity). 

Sequence Annotation

 ACT_SITE 94 94 Glycyl thioester intermediate (By  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Endoplasmic reticulum; Ligase; Membrane; Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway; Unfolded protein response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 259 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS