| Tag | Content |
|---|
| CPLM ID | CPLM-002750 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Respiratory nitrate reductase 1 alpha chain |
Protein Synonyms/Alias | Nitrate reductase A subunit alpha; Quinol-nitrate oxidoreductase subunit alpha |
Gene Name | narG |
Gene Synonyms/Alias | bisD; narC; b1224; JW1215 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 455 | LENVLLHKLPVKRLQ | acetylation | [1] | | 686 | NPVDYTVKSLKEGSI | acetylation | [1] | | 730 | KGHEFMLKYLLGTEH | acetylation | [1] | | 742 | TEHGIQGKDLGQQGG | acetylation | [1] | | 909 | SIGPLMEKIGNGGKG | acetylation | [1] | | 1229 | FVVVRKMKNIDWLDG | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction. |
Sequence Annotation | DOMAIN 43 107 4Fe-4S Mo/W bis-MGD-type.
METAL 50 50 Iron-sulfur (4Fe-4S); via pros nitrogen.
METAL 54 54 Iron-sulfur (4Fe-4S).
METAL 58 58 Iron-sulfur (4Fe-4S).
METAL 93 93 Iron-sulfur (4Fe-4S).
METAL 223 223 Molybdenum. |
Keyword | 3D-structure; 4Fe-4S; Cell membrane; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Reference proteome; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1247 AA |
Protein Sequence | MSKFLDRFRY FKQKGETFAD GHGQLLNTNR DWEDGYRQRW QHDKIVRSTH GVNCTGSCSW 60
KIYVKNGLVT WETQQTDYPR TRPDLPNHEP RGCPRGASYS WYLYSANRLK YPMMRKRLMK 120
MWREAKALHS DPVEAWASII EDADKAKSFK QARGRGGFVR SSWQEVNELI AASNVYTIKN 180
YGPDRVAGFS PIPAMSMVSY ASGARYLSLI GGTCLSFYDW YCDLPPASPQ TWGEQTDVPE 240
SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTVA VTPDYAEIAK LCDLWLAPKQ 300
GTDAAMALAM GHVMLREFHL DNPSQYFTDY VRRYTDMPML VMLEERDGYY AAGRMLRAAD 360
LVDALGQENN PEWKTVAFNT NGEMVAPNGS IGFRWGEKGK WNLEQRDGKT GEETELQLSL 420
LGSQDEIAEV GFPYFGGDGT EHFNKVELEN VLLHKLPVKR LQLADGSTAL VTTVYDLTLA 480
NYGLERGLND VNCATSYDDV KAYTPAWAEQ ITGVSRSQII RIAREFADNA DKTHGRSMII 540
VGAGLNHWYH LDMNYRGLIN MLIFCGCVGQ SGGGWAHYVG QEKLRPQTGW QPLAFALDWQ 600
RPARHMNSTS YFYNHSSQWR YETVTAEELL SPMADKSRYT GHLIDFNVRA ERMGWLPSAP 660
QLGTNPLTIA GEAEKAGMNP VDYTVKSLKE GSIRFAAEQP ENGKNHPRNL FIWRSNLLGS 720
SGKGHEFMLK YLLGTEHGIQ GKDLGQQGGV KPEEVDWQDN GLEGKLDLVV TLDFRLSSTC 780
LYSDIILPTA TWYEKDDMNT SDMHPFIHPL SAAVDPAWEA KSDWEIYKAI AKKFSEVCVG 840
HLGKETDIVT LPIQHDSAAE LAQPLDVKDW KKGECDLIPG KTAPHIMVVE RDYPATYERF 900
TSIGPLMEKI GNGGKGIAWN TQSEMDLLRK LNYTKAEGPA KGQPMLNTAI DAAEMILTLA 960
PETNGQVAVK AWAALSEFTG RDHTHLALNK EDEKIRFRDI QAQPRKIISS PTWSGLEDEH 1020
VSYNAGYTNV HELIPWRTLS GRQQLYQDHQ WMRDFGESLL VYRPPIDTRS VKEVIGQKSN 1080
GNQEKALNFL TPHQKWGIHS TYSDNLLMLT LGRGGPVVWL SEADAKDLGI ADNDWIEVFN 1140
SNGALTARAV VSQRVPAGMT MMYHAQERIV NLPGSEITQQ RGGIHNSVTR ITPKPTHMIG 1200
GYAHLAYGFN YYGTVGSNRD EFVVVRKMKN IDWLDGEGND QVQESVK 1247 |
Gene Ontology | GO:0031224; C:intrinsic to membrane; IDA:EcoCyc. GO:0009325; C:nitrate reductase complex; IEA:InterPro. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc. GO:0009055; F:electron carrier activity; IPI:EcoCyc. GO:0030151; F:molybdenum ion binding; IDA:EcoCyc. GO:0008940; F:nitrate reductase activity; IEA:EC. GO:0016651; F:oxidoreductase activity, acting on NAD(P)H; IBA:RefGenome. GO:0009061; P:anaerobic respiration; IEP:EcoCyc. GO:0017004; P:cytochrome complex assembly; IDA:EcoCyc. GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
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PROSITE | |
PRINTS | |