UniProt Accession

 FUS3_YEAST; P16892; D6VPY4 

Genbank Protein ID

 M31132; X69572; X68577; Z35777; AY693096; BK006936 

Genbank Nucleotide ID

 AAA34613.1; CAA49292.1; CAA48569.1; CAA84835.1; AAT93115.1; DAA07104.1 

Protein Name

 Mitogen-activated protein kinase FUS3 

Protein Synonyms/Alias

 MAP kinase FUS3 

Gene Name

 FUS3 

Gene Synonyms/Alias

 DAC2; YBL016W; YBL03.21; YBL0303 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
345	LTTKDLKKLIWNEIF	ubiquitination	[1]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Together with closely related KSS1, FUS3 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated FUS3 activates the mating but suppresses the filamentation pathway, whereas activated KSS1 activates both pathways. Pheromone-activated FUS3 functions by inhibiting the binding of the transcriptional activator STE12 to filamentation specific genes while inducing its binding to and activity at mating specific genes. Non-activated FUS3 has a repressive effect on STE12 transcriptional activity. KSS1 can partially compensate for the lack of FUS3 but mating efficiency is reduced and the filamentation program is partially activated upon pheromone signaling. FUS3 phosphorylates STE7, STE5, FAR1, DIG1, DIG2 and STE12. 

Sequence Annotation

 DOMAIN 13 309 Protein kinase.
 NP_BIND 19 27 ATP (By similarity).
 MOTIF 180 182 TXY.
 ACT_SITE 137 137 Proton acceptor (By similarity).
 BINDING 42 42 ATP (By similarity).
 MOD_RES 180 180 Phosphothreonine.
 MOD_RES 182 182 Phosphotyrosine.  

Keyword

 3D-structure; ATP-binding; Cell cycle; Cell division; Complete proteome; Conjugation; Cytoplasm; Kinase; Mitosis; Nucleotide-binding; Nucleus; Periplasm; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 353 AA 

Protein Sequence

Gene Ontology

 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004707; F:MAP kinase activity; IDA:SGD.
 GO:0007050; P:cell cycle arrest; IMP:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0001403; P:invasive growth in response to glucose limitation; IMP:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0043409; P:negative regulation of MAPK cascade; IPI:SGD.
 GO:0010526; P:negative regulation of transposition, RNA-mediated; IMP:SGD.
 GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IDA:SGD. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR003527; MAP_kinase_CS.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS01351; MAPK
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS