CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018783
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Amyloid protein-binding protein 2 
Protein Synonyms/Alias
 Amyloid beta precursor protein-binding protein 2; APP-BP2; Protein interacting with APP tail 1 
Gene Name
 APPBP2 
Gene Synonyms/Alias
 KIAA0228; PAT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
178HVRNGNCKYHLGEETubiquitination[1]
195LAQTYMDKLSKHGQQubiquitination[2]
243ITAGLPVKVVVDVLRubiquitination[2]
379HLLLASSKRVKALILubiquitination[1, 2, 3]
396IAIDCHNKETEQRLLubiquitination[3]
419SSLQLAKKAFGEFNVubiquitination[1]
430EFNVQTAKHYGNLGRubiquitination[1, 4, 5, 6]
446YQSMRKFKEAEEMHIubiquitination[1]
504RSIAIGKKLFGEGYSubiquitination[1]
522YDYRGLIKLYNSIGNubiquitination[1, 4, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May play a role in intracellular protein transport. May be involved in the translocation of APP along microtubules toward the cell surface. 
Sequence Annotation
 REPEAT 50 83 TPR 1.
 REPEAT 120 153 TPR 2.
 REPEAT 206 239 TPR 3.
 REPEAT 288 321 TPR 4.
 REPEAT 333 367 TPR 5.
 REPEAT 429 462 TPR 6.
 REPEAT 471 505 TPR 7.
 REPEAT 514 547 TPR 8.  
Keyword
 Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Microtubule; Nucleus; Polymorphism; Protein transport; Reference proteome; Repeat; TPR repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MAAVELEWIP ETLYNTAISA VVDNYIRSRR DIRSLPENIQ FDVYYKLYQQ GRLCQLGSEF 60
CELEVFAKVL RALDKRHLLH HCFQALMDHG VKVASVLAYS FSRRCSYIAE SDAAVKEKAI 120
QVGFVLGGFL SDAGWYSDAE KVFLSCLQLC TLHDEMLHWF RAVECCVRLL HVRNGNCKYH 180
LGEETFKLAQ TYMDKLSKHG QQANKAALYG ELCALLFAKS HYDEAYKWCI EAMKEITAGL 240
PVKVVVDVLR QASKACVVKR EFKKAEQLIK HAVYLARDHF GSKHPKYSDT LLDYGFYLLN 300
VDNICQSVAI YQAALDIRQS VFGGKNIHVA TAHEDLAYSS YVHQYSSGKF DNALFHAERA 360
IGIITHILPE DHLLLASSKR VKALILEEIA IDCHNKETEQ RLLQEAHDLH LSSLQLAKKA 420
FGEFNVQTAK HYGNLGRLYQ SMRKFKEAEE MHIKAIQIKE QLLGQEDYEV ALSVGHLASL 480
YNYDMNQYEN AEKLYLRSIA IGKKLFGEGY SGLEYDYRGL IKLYNSIGNY EKVFEYHNVL 540
SNWNRLRDRQ YSVTDALEDV STSPQSTEEV VQSFLISQNV EGPSC 585 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; IDA:MGI.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005875; C:microtubule associated complex; TAS:ProtInc.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0003777; F:microtubule motor activity; TAS:ProtInc.
 GO:0006886; P:intracellular protein transport; TAS:ProtInc. 
Interpro
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
  
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS