UniProt Accession

 TBP_HUMAN; P20226; B4E3B3; F5H869; Q16845; Q6IBM6; Q9UC02 

Genbank Protein ID

 X54993; M55654; M34960; AK304648; CR456776; AL031259 

Genbank Nucleotide ID

 CAA38736.1; AAA36731.1; AAC03409.1; BAG65425.1; CAG33057.1; CAA20286.1 

Protein Name

 TATA-box-binding protein 

Protein Synonyms/Alias

 TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit 

Gene Name

 TBP 

Gene Synonyms/Alias

 GTF2D1; TF2D; TFIID 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
181	LGCKLDLKTIALRAR	ubiquitination	[1, 2, 3, 4, 5]
225	KMVCTGAKSEEQSRL	ubiquitination	[4, 6]
243	KYARVVQKLGFPAKF	ubiquitination	[1, 4, 5]
249	QKLGFPAKFLDFKIQ	ubiquitination	[1, 4, 5, 7]
265	MVGSCDVKFPIRLEG	ubiquitination	[6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. 

Sequence Annotation

 REPEAT 165 241 1.
 REPEAT 255 332 2.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Disease mutation; DNA-binding; Host-virus interaction; Neurodegeneration; Nucleus; Polymorphism; Reference proteome; Repeat; Spinocerebellar ataxia; Transcription; Transcription regulation; Triplet repeat expansion. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 339 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0001939; C:female pronucleus; IEA:Compara.
 GO:0001940; C:male pronucleus; IEA:Compara.
 GO:0005719; C:nuclear euchromatin; IDA:BHF-UCL.
 GO:0005672; C:transcription factor TFIIA complex; IDA:BHF-UCL.
 GO:0005669; C:transcription factor TFIID complex; IDA:UniProtKB.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0007283; P:spermatogenesis; IEA:Compara.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006383; P:transcription from RNA polymerase III promoter; IDA:MGI.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IC:UniProtKB.
 GO:0022415; P:viral reproductive process; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR011989; ARM-like.
 IPR012295; Beta2_adaptin/TBP_C_dom.
 IPR000814; TBP. 

Pfam

 PF00352; TBP 

SMART

  

PROSITE

 PS00351; TFIID 

PRINTS

 PR00686; TIFACTORIID.