CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029369
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase 25 
Protein Synonyms/Alias
 cDNA FLJ78718 
Gene Name
 STK25 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
65RKIHRDIKAANVLLSubiquitination[1, 2, 3]
78LSEQGDVKLADFGVAubiquitination[2]
94QLTDTQIKRNTFVGTubiquitination[2, 3, 4]
199FITRYTKKTSFLTELubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 349 AA 
Protein Sequence
MAHLRGFANQ STKLWIIMEY LGGGSALDLL KPGPLEETYI ATILREILKG LDYLHSERKI 60
HRDIKAANVL LSEQGDVKLA DFGVAGQLTD TQIKRNTFVG TPFWMAPEVI KQSAYDFKAD 120
IWSLGITAIE LAKGEPPNSD LHPMRVLFLI PKNSPPTLEG QHSKPFKEFV EACLNKDPRF 180
RPTAKELLKH KFITRYTKKT SFLTELIDRY KRWKSEGHGE ESSSEDSDID GEAEDGEQGP 240
IWTFPPTIRP SPHSKLHKGT ALHSSQKPAE PVKRQPRSQC LSTLVRPVFG ELKEKHKQSG 300
GSVGALEELE NAFSLAEESC PGISDKLMVH LVERVQRFSH NRNHLTSTR 349 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004672; F:protein kinase activity; IEA:InterPro. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM 
PRINTS