UniProt Accession

 EHMT1_MOUSE; Q5DW34; A2AIS3; A2AIS4; Q5EBR1; Q6PGM0 

Genbank Protein ID

 AB205007; AL732525; AL732525; AL732525; BC056938; BC089302 

Genbank Nucleotide ID

 BAD90007.1; CAM22112.1; CAM22113.1; CAM22114.1; AAH56938.1; AAH89302.1 

Protein Name

 Histone-lysine N-methyltransferase EHMT1 

Protein Synonyms/Alias

 Euchromatic histone-lysine N-methyltransferase 1; Eu-HMTase1; G9a-like protein 1; GLP; GLP1; Lysine N-methyltransferase 1D 

Gene Name

 Ehmt1 

Gene Synonyms/Alias

 Euhmtase1; Glp; Kmt1d 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
825	DAVKYLIKAGAQVDP	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. 

Sequence Annotation

 REPEAT 735 764 ANK 1.
 REPEAT 770 799 ANK 2.
 REPEAT 803 832 ANK 3.
 REPEAT 836 866 ANK 4.
 REPEAT 870 899 ANK 5.
 REPEAT 903 932 ANK 6.
 REPEAT 936 965 ANK 7.
 REPEAT 969 1002 ANK 8.
 DOMAIN 1058 1121 Pre-SET.
 DOMAIN 1124 1241 SET.
 REGION 903 905 Histone H3K9me binding (By similarity).
 REGION 1134 1136 S-adenosyl-L-methionine binding (By
 REGION 1160 1179 Interaction with histone H3 (By
 REGION 1198 1199 S-adenosyl-L-methionine binding (By
 REGION 1240 1243 Interaction with histone H3 (By
 METAL 1060 1060 Zinc 1 (By similarity).
 METAL 1060 1060 Zinc 2 (By similarity).
 METAL 1062 1062 Zinc 1 (By similarity).
 METAL 1066 1066 Zinc 1 (By similarity).
 METAL 1066 1066 Zinc 3 (By similarity).
 METAL 1071 1071 Zinc 1 (By similarity).
 METAL 1073 1073 Zinc 2 (By similarity).
 METAL 1103 1103 Zinc 2 (By similarity).
 METAL 1103 1103 Zinc 3 (By similarity).
 METAL 1107 1107 Zinc 2 (By similarity).
 METAL 1109 1109 Zinc 3 (By similarity).
 METAL 1113 1113 Zinc 3 (By similarity).
 METAL 1201 1201 Zinc 4 (By similarity).
 METAL 1254 1254 Zinc 4 (By similarity).
 METAL 1256 1256 Zinc 4 (By similarity).
 METAL 1261 1261 Zinc 4 (By similarity).
 BINDING 1153 1153 Histone H3K9me (By similarity).
 BINDING 1171 1171 S-adenosyl-L-methionine (By similarity).
 BINDING 1255 1255 S-adenosyl-L-methionine; via amide  

Keyword

 Alternative splicing; ANK repeat; Chromatin regulator; Chromosome; Complete proteome; Metal-binding; Methyltransferase; Nucleus; Reference proteome; Repeat; S-adenosyl-L-methionine; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1296 AA 

Protein Sequence

Gene Ontology

 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0046976; F:histone methyltransferase activity (H3-K27 specific); IDA:UniProtKB.
 GO:0046974; F:histone methyltransferase activity (H3-K9 specific); IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006306; P:DNA methylation; IDA:UniProtKB.
 GO:0009790; P:embryo development; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:MGI.
 GO:0018027; P:peptidyl-lysine dimethylation; IMP:UniProtKB.
 GO:0018026; P:peptidyl-lysine monomethylation; IMP:UniProtKB. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR007728; Pre-SET_dom.
 IPR003606; Pre-SET_Zn-bd_sub.
 IPR001214; SET_dom. 

Pfam

 PF00023; Ank
 PF12796; Ank_2
 PF05033; Pre-SET
 PF00856; SET 

SMART

 SM00248; ANK
 SM00468; PreSET
 SM00317; SET 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50867; PRE_SET
 PS50280; SET 

PRINTS

 PR01415; ANKYRIN.