CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012002
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TNF receptor-associated factor 3 
Protein Synonyms/Alias
 CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1 
Gene Name
 TRAF3 
Gene Synonyms/Alias
 CAP1; CRAF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99QESIVKDKVFKDNCCubiquitination[1]
351WEEADSMKSSVESLQubiquitination[1, 2]
369TELESVDKSAGQVARubiquitination[1, 2, 3, 4]
513RHLGDAFKPDPNSSSubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. 
Sequence Annotation
 DOMAIN 415 560 MATH.
 ZN_FING 68 77 RING-type.
 ZN_FING 135 190 TRAF-type 1.
 ZN_FING 191 249 TRAF-type 2.
 CROSSLNK 107 107 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 156 156 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Apoptosis; Coiled coil; Complete proteome; Cytoplasm; Disease mutation; Endosome; Immunity; Isopeptide bond; Ligase; Metal-binding; Mitochondrion; Polymorphism; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 568 AA 
Protein Sequence
MESSKKMDSP GALQTNPPLK LHTDRSAGTP VFVPEQGGYK EKFVKTVEDK YKCEKCHLVL 60
CSPKQTECGH RFCESCMAAL LSSSSPKCTA CQESIVKDKV FKDNCCKREI LALQIYCRNE 120
SRGCAEQLML GHLLVHLKND CHFEELPCVR PDCKEKVLRK DLRDHVEKAC KYREATCSHC 180
KSQVPMIALQ KHEDTDCPCV VVSCPHKCSV QTLLRSELSA HLSECVNAPS TCSFKRYGCV 240
FQGTNQQIKA HEASSAVQHV NLLKEWSNSL EKKVSLLQNE SVEKNKSIQS LHNQICSFEI 300
EIERQKEMLR NNESKILHLQ RVIDSQAEKL KELDKEIRPF RQNWEEADSM KSSVESLQNR 360
VTELESVDKS AGQVARNTGL LESQLSRHDQ MLSVHDIRLA DMDLRFQVLE TASYNGVLIW 420
KIRDYKRRKQ EAVMGKTLSL YSQPFYTGYF GYKMCARVYL NGDGMGKGTH LSLFFVIMRG 480
EYDALLPWPF KQKVTLMLMD QGSSRRHLGD AFKPDPNSSS FKKPTGEMNI ASGCPVFVAQ 540
TVLENGTYIK DDTIFIKVIV DTSDLPDP 568 
Gene Ontology
 GO:0035631; C:CD40 receptor complex; ISS:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
 GO:0009898; C:internal side of plasma membrane; ISS:BHF-UCL.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0004842; F:ubiquitin-protein ligase activity; TAS:Reactome.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006917; P:induction of apoptosis; TAS:ProtInc.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IMP:UniProtKB.
 GO:0032480; P:negative regulation of type I interferon production; TAS:Reactome.
 GO:0050688; P:regulation of defense response to virus; ISS:UniProtKB.
 GO:0032648; P:regulation of interferon-beta production; ISS:UniProtKB.
 GO:0030162; P:regulation of proteolysis; IMP:UniProtKB.
 GO:0008063; P:Toll signaling pathway; IEA:InterPro.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IMP:UniProtKB. 
Interpro
 IPR002083; MATH.
 IPR013323; SIAH-type.
 IPR012227; TNF_rcpt--assoc_TRAF.
 IPR008974; TRAF-like.
 IPR027128; TRAF3.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS.
 IPR001293; Znf_TRAF. 
Pfam
 PF00917; MATH 
SMART
 SM00061; MATH
 SM00184; RING 
PROSITE
 PS50144; MATH
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2
 PS50145; ZF_TRAF 
PRINTS