UniProt Accession

 RNF5_MOUSE; O35445 

Genbank Protein ID

 AF030001; BC016449 

Genbank Nucleotide ID

 AAB82008.1; AAH16449.1 

Protein Name

 E3 ubiquitin-protein ligase RNF5 

Protein Synonyms/Alias

 RING finger protein 5 

Gene Name

 Rnf5 

Gene Synonyms/Alias

 Ng2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
68	RQECPVCKAGISREK	ubiquitination	[1]
75	KAGISREKVVPLYGR	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin- conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150 'leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses (By similarity). 

Sequence Annotation

 ZN_FING 27 68 RING-type.
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; Complete proteome; Endoplasmic reticulum; Ligase; Membrane; Metal-binding; Mitochondrion; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 180 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0071712; P:ER-associated misfolded protein catabolic process; ISS:UniProtKB.
 GO:0070936; P:protein K48-linked ubiquitination; IEA:Compara.
 GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB. 

Interpro

 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 

Pfam

  

SMART

 SM00184; RING 

PROSITE

 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS