CPLM-020394

Genbank Nucleotide ID

Kinetochore protein Nuf2

Protein Synonyms/Alias

hNuf2; hNuf2R; hsNuf2; Cell division cycle-associated protein 1

Homo sapiens (Human)

Position	Peptide	Type	References
21	IVIHIRNKILTGADG	ubiquitination	[1]
29	ILTGADGKNLTKNDL	ubiquitination	[1, 2, 3]
33	ADGKNLTKNDLYPNP	ubiquitination	[1]
113	TADILCPKAKRTSRF	ubiquitination	[1, 4]
115	DILCPKAKRTSRFLS	ubiquitination	[1]
151	QYKSSADKMQQLNAA	ubiquitination	[1]
165	AHQEALMKLERLDSV	ubiquitination	[2]
201	LNQDFHQKTIVLQEG	ubiquitination	[2]
212	LQEGNSQKKSNISEK	ubiquitination	[1]
213	QEGNSQKKSNISEKT	ubiquitination	[1]
234	KLSVVSLKEIQESLK	ubiquitination	[2]
241	KEIQESLKTKIVDSP	ubiquitination	[1]
243	IQESLKTKIVDSPEK	ubiquitination	[1]
250	KIVDSPEKLKNYKEK	ubiquitination	[1]
299	EVQLYQKKIQDLSDN	ubiquitination	[1, 5]
309	DLSDNREKLASILKE	ubiquitination	[1]
315	EKLASILKESLNLED	ubiquitination	[1, 2]
332	ESDESELKKLKTEEN	ubiquitination	[2]
333	SDESELKKLKTEENS	ubiquitination	[1]
335	ESELKKLKTEENSFK	ubiquitination	[1]
342	KTEENSFKRLMIVKK	ubiquitination	[1]
379	TVIEDCNKVQEKRGA	ubiquitination	[1]
383	DCNKVQEKRGAVYER	ubiquitination	[1]
400	TINQEIQKIKLGIQQ	ubiquitination	[1]
402	NQEIQKIKLGIQQLK	ubiquitination	[1]
409	KLGIQQLKDAAEREK	ubiquitination	[1, 2]
418	AAEREKLKSQEIFLN	ubiquitination	[1]
427	QEIFLNLKTALEKYH	ubiquitination	[1]
432	NLKTALEKYHDGIEK	ubiquitination	[1]
439	KYHDGIEKAAEDSYA	ubiquitination	[1]
447	AAEDSYAKIDEKTAE	ubiquitination	[1]
451	SYAKIDEKTAELKRK	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

REGION 1 385 Interaction with the N-terminus of NDC80.
REGION 386 464 Interaction with the C-terminus of NDC80

3D-structure; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0031262; C:Ndc80 complex; IDA:UniProtKB.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0007059; P:chromosome segregation; NAS:UniProtKB.
GO:0007067; P:mitosis; IEA:UniProtKB-KW.
GO:0000278; P:mitotic cell cycle; TAS:Reactome.

IPR005549; Kinetochore_Nuf2.