CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007459
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ETS translocation variant 3 
Protein Synonyms/Alias
 ETS domain transcriptional repressor PE1; PE-1; Mitogenic Ets transcriptional suppressor 
Gene Name
 ETV3 
Gene Synonyms/Alias
 METS; PE1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10AGCSIVEKPEGGGGYubiquitination[1]
25QFPDWAYKTESSPGSubiquitination[1, 2, 3]
80ARLWGRRKCKPQMNYubiquitination[1]
82LWGRRKCKPQMNYDKubiquitination[1, 3]
89KPQMNYDKLSRALRYubiquitination[1]
114KGKRFTYKFNFNKLVubiquitination[1]
188SSNGTDRKTELSELEubiquitination[1]
224GGGIGHQKRKPDIMLubiquitination[1, 3]
388KVEPASEKDPESLRQacetylation[4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 
Functional Description
 Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity. 
Sequence Annotation
 DNA_BIND 35 116 ETS.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 388 388 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 512 AA 
Protein Sequence
MKAGCSIVEK PEGGGGYQFP DWAYKTESSP GSRQIQLWHF ILELLQKEEF RHVIAWQQGE 60
YGEFVIKDPD EVARLWGRRK CKPQMNYDKL SRALRYYYNK RILHKTKGKR FTYKFNFNKL 120
VMPNYPFINI RSSGVVPQSA PPVPTASSRF HFPPLDTHSP TNDVQPGRFS ASSLTASGQE 180
SSNGTDRKTE LSELEDGSAA DWRRGVDPVS SRNAIGGGGI GHQKRKPDIM LPLFARPGMY 240
PDPHSPFAVS PIPGRGGVLN VPISPALSLT PTIFSYSPSP GLSPFTSSSC FSFNPEEMKH 300
YLHSQACSVF NYHLSPRTFP RYPGLMVPPL QCQMHPEEST QFSIKLQPPP VGRKNRERVE 360
SSEESAPVTT PTMASIPPRI KVEPASEKDP ESLRQSAREK EEHTQEEGTV PSRTIEEEKG 420
TIFARPAAPP IWPSVPISTP SGEPLEVTED SEDRPGKEPS APEKKEDALM PPKLRLKRRW 480
NDDPEARELS KSGKFLWNGS GPQGLATAAA DA 512 
Gene Ontology
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0017053; C:transcriptional repressor complex; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000418; Ets_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00178; Ets 
SMART
 SM00413; ETS 
PROSITE
 PS00345; ETS_DOMAIN_1
 PS00346; ETS_DOMAIN_2
 PS50061; ETS_DOMAIN_3 
PRINTS
 PR00454; ETSDOMAIN.