CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013333
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hydroxyacid-oxoacid transhydrogenase, mitochondrial 
Protein Synonyms/Alias
 HOT; Alcohol dehydrogenase iron-containing protein 1; ADHFe1 
Gene Name
 Adhfe1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
62RYGAGVTKEVGMDLQacetylation[1]
172NAPIGKGKPVTVPLKacetylation[1]
179KPVTVPLKPLIAVPTacetylation[1]
292HALRIVAKYLKRAVRacetylation[1]
295RIVAKYLKRAVRNPDacetylation[1]
445LPQERVTKLAPRAQSacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2- hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; Mitochondrion; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 467 AA 
Protein Sequence
MAAAARARVT HLLRHLQSTA CQCPTHSHTY SQVPGLSPSG KTTDYAFEMA VSNIRYGAGV 60
TKEVGMDLQN MGAKNVCLMT DKNLSQLPPV QIVMDSLSKN GISFQVYDNV RVEPTDGSFM 120
DAIEFAKKGA FDAYVAVGGG STMDTCKAAN LYACSPHSEF LDYVNAPIGK GKPVTVPLKP 180
LIAVPTTSGT GSETTGVAIF DYEHLKVKTG IASRAIKPTL GLVDPLHTLH MPCQVVANSG 240
FDVLCHALES YTAIPYSMRS PCPSNPIQRP AYQGSNPISD IWAVHALRIV AKYLKRAVRN 300
PDDLEARSSM HLASAFAGIG FGNAGVHLCH GMSYPISGLV KTYKAKEYNV DHPLVPHGLS 360
VVLTSPAVFT FTAQMFPERH LETAEILGAN IRTAKIQDAG PVLADALRKF LFDLNVDDGL 420
AALGYSKDDI PSLVKGTLPQ ERVTKLAPRA QSEEDLSALF EASMKLY 467 
Gene Ontology
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0047988; F:hydroxyacid-oxoacid transhydrogenase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0015993; P:molecular hydrogen transport; ISS:UniProtKB. 
Interpro
 IPR001670; ADH_Fe. 
Pfam
 PF00465; Fe-ADH 
SMART
  
PROSITE
 PS00913; ADH_IRON_1
 PS00060; ADH_IRON_2 
PRINTS