CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032196
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial 
Protein Synonyms/Alias
 cDNA, FLJ78862, highly similar to Isocitrate dehydrogenase; cDNA, FLJ78950, highly similar to Isocitrate dehydrogenase 
Gene Name
 IDH3A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MNLLLRKTFDLYANubiquitination[1]
68LITEGASKRIAEFAFubiquitination[1]
91SNVTAVHKANIMRMSubiquitination[1]
105SDGLFLQKCREVAESubiquitination[1]
114REVAESCKDIKFNEMubiquitination[1]
227AACFATIKDGKSLTKubiquitination[1]
234KDGKSLTKDLGGNAKacetylation[2, 3]
234KDGKSLTKDLGGNAKubiquitination[1]
241KDLGGNAKCSDFTEEubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 257 AA 
Protein Sequence
MNLLLRKTFD LYANVRPCVS IEGYKTPYTD VNIVTIRENT EGEYSGIEHV IVDGVVQSIK 60
LITEGASKRI AEFAFEYARN NHRSNVTAVH KANIMRMSDG LFLQKCREVA ESCKDIKFNE 120
MYLDTVCLNM VQDPSQFDVL VMPNLYGDIL SDLCAGLIGG LGVTPSGNIG ANGVAIFESV 180
HGTAPDIAGK DMANPTALLL SAVMMLRHMG LFDHAARIEA ACFATIKDGK SLTKDLGGNA 240
KCSDFTEEIC RRVKDLD 257 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro. 
Interpro
 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR001804; Isocitrate/isopropylmalate_DH.
 IPR024084; IsoPropMal-DH-like_dom. 
Pfam
 PF00180; Iso_dh 
SMART
  
PROSITE
 PS00470; IDH_IMDH 
PRINTS