UniProt Accession

 DNJC3_HUMAN; Q13217; Q86WT9; Q8N4N2 

Genbank Protein ID

 U28424; AY795482; AL138955; BC047936 

Genbank Nucleotide ID

 AAC50502.1; AAV40838.1; CAH70090.1; AAH47936.2 

Protein Name

 DnaJ homolog subfamily C member 3 

Protein Synonyms/Alias

 Endoplasmic reticulum DnaJ protein 6; ERdj6; Interferon-induced, double-stranded RNA-activated protein kinase inhibitor; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58 

Gene Name

 DNAJC3 

Gene Synonyms/Alias

 P58IPK; PRKRI 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
96	AALPDLTKVIQLKMD	ubiquitination	[1]
133	DDFKKVLKSNPSENE	ubiquitination	[1, 2]
142	NPSENEEKEAQSQLI	ubiquitination	[2]
150	EAQSQLIKSDEMQRL	ubiquitination	[2]
212	RKAISDLKAASKLKN	ubiquitination	[1]
256	LKLDQDHKRCFAHYK	ubiquitination	[2]
290	RYTDATSKYESVMKT	ubiquitination	[1, 2]
344	PDNVNALKDRAEAYL	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. 

Sequence Annotation

 REPEAT 37 70 TPR 1.
 REPEAT 72 104 TPR 2.
 REPEAT 105 138 TPR 3.
 REPEAT 154 187 TPR 4.
 REPEAT 189 221 TPR 5.
 REPEAT 222 255 TPR 6.
 REPEAT 268 301 TPR 7.
 REPEAT 306 339 TPR 8.
 REPEAT 340 373 TPR 9.
 DOMAIN 394 462 J.
 REGION 375 393 Flexible linker.
 DISULFID 248 258
 DISULFID 313 329  

Keyword

 3D-structure; Antiviral defense; Chaperone; Complete proteome; Disulfide bond; Endoplasmic reticulum; Reference proteome; Repeat; Signal; TPR repeat; Unfolded protein response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 504 AA 

Protein Sequence

Gene Ontology

 GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
 GO:0004860; F:protein kinase inhibitor activity; TAS:ProtInc.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW. 

Interpro

 IPR001623; DnaJ_domain.
 IPR026901; DNAJC3.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 

Pfam

 PF00226; DnaJ
 PF00515; TPR_1 

SMART

 SM00271; DnaJ
 SM00028; TPR 

PROSITE

 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS

 PR00625; JDOMAIN.