UniProt Accession

 EHMT2_HUMAN; Q96KQ7; B0UZY2; Q14349; Q5JP83; Q5JQ92; Q5JQA1; Q5JQG3; Q6PK06; Q96MH5; Q96QD0; Q9UQL8; Q9Y331 

Genbank Protein ID

 AJ315532; AK056936; AF134726; AF134726; BA000025; BA000025; AL662834; AL662834; AL671762; AL671762; AL844853; AL844853; CR388219; CR388202; CR388219; CR388202; CR936237; CR759784; CR388202; CR388219; CR388202; CR388219; CH471081; BC002686; BC009351; BC018718; BC020970; X69838 

Genbank Nucleotide ID

 CAC86666.1; BAB71314.1; AAD21811.1; AAD21812.1; BAB63294.1; BAB63295.1; CAI17747.2; CAI17748.1; CAI18224.2; CAI18226.2; CAI41852.1; CAI41853.1; CAQ07473.1; CAQ07473.1; CAQ07474.1; CAQ07474.1; CAQ09159.1; CAQ09311.1; CAQ09508.1; CAQ09508.1; CAQ09509.1; CAQ09509.1; EAX03542.1; AAH02686.2; AAH09351.1; AAH18718.1; AAH20970.2; CAA49491.1 

Protein Name

 Histone-lysine N-methyltransferase EHMT2 

Protein Synonyms/Alias

 Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Lysine N-methyltransferase 1C; Protein G9a 

Gene Name

 EHMT2 

Gene Synonyms/Alias

 BAT8; C6orf30; G9A; KMT1C; NG36 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
185	PKVHRARKTMSKPGN	methylation	[1, 2]
882	ANPELRNKEGDTAWD	ubiquitination	[3]
1047	LQLYRTAKMGWGVRA	ubiquitination	[4]

Reference

 [1] Protein lysine methyltransferase G9a acts on non-histone targets.
 Rathert P, Dhayalan A, Murakami M, Zhang X, Tamas R, Jurkowska R, Komatsu Y, Shinkai Y, Cheng X, Jeltsch A.
 Nat Chem Biol. 2008 Jun;4(6):344-6. [PMID: 18438403]
 [2] Update on activities at the Universal Protein Resource (UniProt) in 2013.
 e="String">UniProt Consortium.
 Nucleic Acids Res. 2013 Jan;41(Database issue):D43-7. [PMID: 23161681]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. 

Sequence Annotation

 REPEAT 649 678 ANK 1.
 REPEAT 684 713 ANK 2.
 REPEAT 717 746 ANK 3.
 REPEAT 750 780 ANK 4.
 REPEAT 784 813 ANK 5.
 REPEAT 817 846 ANK 6.
 REPEAT 850 879 ANK 7.
 DOMAIN 972 1035 Pre-SET.
 DOMAIN 1038 1155 SET.
 DOMAIN 1164 1180 Post-SET.
 REGION 817 819 Histone H3K9me binding (By similarity).
 REGION 1048 1050 S-adenosyl-L-methionine binding.
 REGION 1074 1093 Interaction with histone H3 (By
 REGION 1112 1113 S-adenosyl-L-methionine binding.
 REGION 1154 1157 Interaction with histone H3 (By
 METAL 974 974 Zinc 1.
 METAL 974 974 Zinc 2.
 METAL 976 976 Zinc 1.
 METAL 980 980 Zinc 1.
 METAL 980 980 Zinc 3.
 METAL 985 985 Zinc 1.
 METAL 987 987 Zinc 2.
 METAL 1017 1017 Zinc 2.
 METAL 1017 1017 Zinc 3.
 METAL 1021 1021 Zinc 2.
 METAL 1023 1023 Zinc 3.
 METAL 1027 1027 Zinc 3.
 METAL 1115 1115 Zinc 4.
 METAL 1168 1168 Zinc 4.
 METAL 1170 1170 Zinc 4.
 METAL 1175 1175 Zinc 4.
 BINDING 1067 1067 Histone H3K9me (By similarity).
 BINDING 1085 1085 S-adenosyl-L-methionine.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 140 140 Phosphoserine.
 MOD_RES 173 173 Phosphoserine.
 MOD_RES 185 185 N6,N6,N6-trimethyllysine; by EHMT2;
 MOD_RES 185 185 N6,N6-dimethyllysine; by EHMT2;
 MOD_RES 232 232 Phosphoserine.
 MOD_RES 246 246 Phosphoserine.
 MOD_RES 413 413 Phosphoserine.
 MOD_RES 555 555 Phosphothreonine (By similarity).
 MOD_RES 569 569 Phosphoserine.
 MOD_RES 1204 1204 Phosphoserine.
 MOD_RES 1210 1210 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ANK repeat; Chromatin regulator; Chromosome; Complete proteome; Metal-binding; Methylation; Methyltransferase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; S-adenosyl-L-methionine; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1210 AA 

Protein Sequence

Gene Ontology

 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0046976; F:histone methyltransferase activity (H3-K27 specific); ISS:UniProtKB.
 GO:0046974; F:histone methyltransferase activity (H3-K9 specific); ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006306; P:DNA methylation; ISS:UniProtKB.
 GO:0010424; P:DNA methylation on cytosine within a CG sequence; IEA:Compara.
 GO:0009566; P:fertilization; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0035265; P:organ growth; IEA:Compara.
 GO:0018027; P:peptidyl-lysine dimethylation; IDA:UniProtKB.
 GO:0006275; P:regulation of DNA replication; IMP:UniProtKB.
 GO:0007286; P:spermatid development; IEA:Compara.
 GO:0007130; P:synaptonemal complex assembly; IEA:Compara. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR003616; Post-SET_dom.
 IPR007728; Pre-SET_dom.
 IPR003606; Pre-SET_Zn-bd_sub.
 IPR001214; SET_dom. 

Pfam

 PF00023; Ank
 PF05033; Pre-SET
 PF00856; SET 

SMART

 SM00248; ANK
 SM00508; PostSET
 SM00468; PreSET
 SM00317; SET 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50868; POST_SET
 PS50867; PRE_SET
 PS50280; SET 

PRINTS

 PR01415; ANKYRIN.