UniProt Accession

 MBF1_YEAST; O14467; D6W2Z8; Q7LGJ8; Q86ZS7 

Genbank Protein ID

 AB017593; Z75206; Z75207; AY260886; AY692753; BK006948 

Genbank Nucleotide ID

 BAA33217.1; CAA99527.1; CAA99530.1; AAP21754.1; AAT92772.1; DAA11064.1 

Protein Name

 Multiprotein-bridging factor 1 

Protein Synonyms/Alias

 Suppressor of frameshift mutations protein 13 

Gene Name

 MBF1 

Gene Synonyms/Alias

 SUF13; YOR298C-A 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
106	LATKINEKPTVVNDY	acetylation	[1]
106	LATKINEKPTVVNDY	ubiquitination	[2]
127	PNQQVLSKLERALGV	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Transcriptional coactivator that stimulates GCN4- dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4- bound promoters. 

Sequence Annotation

 DOMAIN 85 139 HTH cro/C1-type.
 DNA_BIND 96 115 H-T-H motif (Potential).
 REGION 41 119 Essential for TBP-binding.
 MOD_RES 143 143 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 151 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IC:SGD.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003713; F:transcription coactivator activity; IDA:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR001387; HTH.
 IPR010982; Lambda_DNA-bd_dom.
 IPR013729; MBF1_N. 

Pfam

 PF01381; HTH_3
 PF08523; MBF1 

SMART

 SM00530; HTH_XRE 

PROSITE

 PS50943; HTH_CROC1 

PRINTS