CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002415
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histidine biosynthesis bifunctional protein HisB 
Protein Synonyms/Alias
 Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD 
Gene Name
 hisB 
Gene Synonyms/Alias
 b2022; JW2004 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
29FQVDRFDKLAFEPGVacetylation[1]
42GVIPELLKLQKAGYKacetylation[1]
250LKIALGDKRGICRFGacetylation[1]
282GRPHLEYKAEFTYQRacetylation[1]
331HRVESLFKAFGRTLRacetylation[1, 2]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
  
Sequence Annotation
 REGION 1 166 Histidinol-phosphatase.
 REGION 167 355 Imidazoleglycerol-phosphate dehydratase.  
Keyword
 Amino-acid biosynthesis; Complete proteome; Cytoplasm; Histidine biosynthesis; Hydrolase; Lyase; Multifunctional enzyme; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 355 AA 
Protein Sequence
MSQKYLFIDR DGTLISEPPS DFQVDRFDKL AFEPGVIPEL LKLQKAGYKL VMITNQDGLG 60
TQSFPQADFD GPHNLMMQIF TSQGVQFDEV LICPHLPADE CDCRKPKVKL VERYLAEQAM 120
DRANSYVIGD RATDIQLAEN MGITGLRYDR ETLNWPMIGE QLTRRDRYAH VVRNTKETQI 180
DVQVWLDREG GSKINTGVGF FDHMLDQIAT HGGFRMEINV KGDLYIDDHH TVEDTGLALG 240
EALKIALGDK RGICRFGFVL PMDECLARCA LDISGRPHLE YKAEFTYQRV GDLSTEMIEH 300
FFRSLSYTMG VTLHLKTKGK NDHHRVESLF KAFGRTLRQA IRVEGDTLPS SKGVL 355 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004401; F:histidinol-phosphatase activity; IDA:EcoCyc.
 GO:0004424; F:imidazoleglycerol-phosphate dehydratase activity; IEA:HAMAP.
 GO:0000105; P:histidine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR023214; HAD-like_dom.
 IPR006549; HAD-SF_hydro_IIIA.
 IPR020566; His_synth_bifunc_HisB.
 IPR005954; HisB_N.
 IPR006543; Histidinol-phos.
 IPR000807; ImidazoleglycerolP_deHydtase.
 IPR020565; ImidazoleglycerP_deHydtase_CS.
 IPR013954; PNK3P.
 IPR020568; Ribosomal_S5_D2-typ_fold. 
Pfam
 PF00475; IGPD
 PF08645; PNK3P 
SMART
  
PROSITE
 PS00954; IGP_DEHYDRATASE_1
 PS00955; IGP_DEHYDRATASE_2 
PRINTS