UniProt Accession

 CYSK_ECOLI; P0ABK5; P11096; P21546 

Genbank Protein ID

 X12615; M21451; U00096; AP009048; M21994 

Genbank Nucleotide ID

 CAA31137.1; AAA23654.1; AAC75467.1; BAA16288.1; AAA24383.1 

Protein Name

 Cysteine synthase A 

Protein Synonyms/Alias

 CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5 

Gene Name

 cysK 

Gene Synonyms/Alias

 cysZ; b2414; JW2407 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
3	*****MSKIFEDNSL	acetylation	[1]
31	GNGRILAKVESRNPS	acetylation	[1]
42	RNPSFSVKCRIGANM	acetylation	[1]
55	NMIWDAEKRGVLKPG	acetylation	[1]
60	AEKRGVLKPGVELVE	acetylation	[1]
87	VAAARGYKLTLTMPE	acetylation	[1]
105	IERRKLLKALGANLV	acetylation	[1]
118	LVLTEGAKGMKGAIQ	acetylation	[1]
121	TEGAKGMKGAIQKAE	acetylation	[1]
126	GMKGAIQKAEEIVAS	acetylation	[1, 2]
137	IVASNPEKYLLLQQF	acetylation	[1, 3]
221	ALAGEEIKPGPHKIQ	acetylation	[1]
226	EIKPGPHKIQGIGAG	acetylation	[1]
242	IPANLDLKLVDKVIG	acetylation	[1]
246	LDLKLVDKVIGITNE	acetylation	[1]
293	EDESFTNKNIVVILP	acetylation	[1]
319	FADLFTEKELQQ***	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

  

Sequence Annotation

 REGION 177 181 Pyridoxal phosphate binding (By
 BINDING 8 8 Allosteric inhibitor (By similarity).
 BINDING 72 72 Pyridoxal phosphate (By similarity).
 BINDING 269 269 Allosteric inhibitor; via amide nitrogen
 BINDING 273 273 Pyridoxal phosphate (By similarity).
 MOD_RES 42 42 N6-(pyridoxal phosphate)lysine (By  

Keyword

 Allosteric enzyme; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Direct protein sequencing; Lyase; Pyridoxal phosphate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 323 AA 

Protein Sequence

Gene Ontology

 GO:0009333; C:cysteine synthase complex; IDA:EcoCyc.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0004124; F:cysteine synthase activity; IDA:EcoCyc.
 GO:0080146; F:L-cysteine desulfhydrase activity; IMP:EcoCyc.
 GO:0050272; F:S-carboxymethylcysteine synthase activity; IEA:EC.
 GO:0016740; F:transferase activity; IDA:EcoliWiki.
 GO:0006535; P:cysteine biosynthetic process from serine; IDA:EcoCyc. 

Interpro

 IPR001216; Cys_synth_BS.
 IPR005856; Cys_synthKM.
 IPR005859; CysK.
 IPR001926; Trp_syn_b_sub_like_PLP_eny_SF. 

Pfam

 PF00291; PALP 

SMART

  

PROSITE

 PS00901; CYS_SYNTHASE 

PRINTS