UniProt Accession

 PKHA8_CANFA; D2KC46; E2R9N6 

Genbank Protein ID

 GU204956 

Genbank Nucleotide ID

 ACZ98822.1 

Protein Name

 Pleckstrin homology domain-containing family A member 8 

Protein Synonyms/Alias

 PH domain-containing family A member 8; Phosphatidylinositol-four-phosphate adapter protein 2; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2 

Gene Name

 PLEKHA8 

Gene Synonyms/Alias

 FAPP2 

Created Date

 July 27, 2013 

Organism

 Canis familiaris (Dog) (Canis lupus familiaris) 

NCBI Taxa ID

 9615 

Lysine Modification

Position	Peptide	Type	References
416	KRGLKFLKGFLTEVK	ubiquitination	[1, 2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Cargo transport protein that is required for apical transport from the trans-Golgi network (TGN). Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4- phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation. 

Sequence Annotation

 DOMAIN 1 93 PH.
 REGION 310 519 Glycolipid transfer protein homology
 MOD_RES 139 139 Phosphothreonine (By similarity).
 MOD_RES 145 145 Phosphoserine (By similarity).
 MOD_RES 153 153 Phosphothreonine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Golgi apparatus; Lipid transport; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 519 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
 GO:0097001; F:ceramide binding; ISS:UniProtKB.
 GO:0051861; F:glycolipid binding; ISS:UniProtKB.
 GO:0017089; F:glycolipid transporter activity; ISS:UniProtKB.
 GO:0070273; F:phosphatidylinositol-4-phosphate binding; ISS:UniProtKB.
 GO:0035621; P:ER to Golgi ceramide transport; ISS:UniProtKB.
 GO:0046836; P:glycolipid transport; IEA:InterPro.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR014830; Glycolipid_transfer_prot_dom.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 

Pfam

 PF08718; GLTP
 PF00169; PH 

SMART

 SM00233; PH 

PROSITE

 PS50003; PH_DOMAIN 

PRINTS