CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019702
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tubulin-folding cofactor B 
Protein Synonyms/Alias
 Cytoskeleton-associated protein 1; Cytoskeleton-associated protein CKAPI; Tubulin-specific chaperone B 
Gene Name
 TBCB 
Gene Synonyms/Alias
 CG22; CKAP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
38SLTIAEFKCKLELLVubiquitination[1]
106EDVSRVEKYTISQEAubiquitination[1, 2, 3, 4, 5, 6, 7]
153AQRLAEEKAQASSIPubiquitination[1, 3, 4, 6]
203RYDEPLGKNDGSVNGubiquitination[1, 3, 6]
219RYFECQAKYGAFVKPacetylation[8]
219RYFECQAKYGAFVKPubiquitination[2, 5, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
 Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth. 
Sequence Annotation
 DOMAIN 183 225 CAP-Gly.
 MOD_RES 65 65 Phosphoserine; by PAK1.
 MOD_RES 98 98 Phosphotyrosine.
 MOD_RES 110 110 Phosphoserine.
 MOD_RES 128 128 Phosphoserine; by PAK1.
 MOD_RES 219 219 N6-acetyllysine.  
Keyword
 Acetylation; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Microtubule; Neurogenesis; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 244 AA 
Protein Sequence
MEVTGVSAPT VTVFISSSLN TFRSEKRYSR SLTIAEFKCK LELLVGSPAS CMELELYGVD 60
DKFYSKLDQE DALLGSYPVD DGCRIHVIDH SGARLGEYED VSRVEKYTIS QEAYDQRQDT 120
VRSFLKRSKL GRYNEEERAQ QEAEAAQRLA EEKAQASSIP VGSRCEVRAA GQSPRRGTVM 180
YVGLTDFKPG YWIGVRYDEP LGKNDGSVNG KRYFECQAKY GAFVKPAVVT VGDFPEEDYG 240
LDEI 244 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; TAS:ProtInc.
 GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW. 
Interpro
 IPR000938; CAP-Gly_domain. 
Pfam
 PF01302; CAP_GLY 
SMART
 SM01052; CAP_GLY 
PROSITE
 PS00845; CAP_GLY_1
 PS50245; CAP_GLY_2 
PRINTS