CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014316
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tumor susceptibility gene 101 protein 
Protein Synonyms/Alias
 ESCRT-I complex subunit TSG101 
Gene Name
 Tsg101 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
238LISAVSDKLRWRMKEubiquitination[1]
244DKLRWRMKEEMDGAQubiquitination[1]
362IDLDVFLKHVRLLSRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator (By similarity). 
Sequence Annotation
 DOMAIN 2 145 UEV.
 DOMAIN 323 391 SB.
 REGION 159 163 Interaction with CEP55 (By similarity).
 MOTIF 321 324 PTAP/PSAP motif.
 MOD_RES 2 2 N-acetylalanine (By similarity).  
Keyword
 Acetylation; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Endosome; Growth regulation; Host-virus interaction; Membrane; Nucleus; Protein transport; Reference proteome; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 391 AA 
Protein Sequence
MAVSESQLKK MMSKYKYRDL TVRQTVNVIA MYKDLKPVLD SYVFNDGSSR ELVNLTGTIP 60
VRYRGNIYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG KHVDANGKIY LPYLHDWKHP 120
RSELLELIQI MIVIFGEEPP VFSRPTVSAS YPPYTATGPP NTSYMPGMPS GISAYPSGYP 180
PNPSGYPGCP YPPAGPYPAT TSSQYPSQPP VTTVGPSRDG TISEDTIRAS LISAVSDKLR 240
WRMKEEMDGA QAELNALKRT EEDLKKGHQK LEEMVTRLDQ EVAEVDKNIE LLKKKDEELS 300
SALEKMENQS ENNDIDEVII PTAPLYKQIL NLYAEENAIE DTIFYLGEAL RRGVIDLDVF 360
LKHVRLLSRK QFQLRALMQK ARKTAGLSDL Y 391 
Gene Ontology
 GO:0005769; C:early endosome; IEA:Compara.
 GO:0000813; C:ESCRT I complex; ISS:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005634; C:nucleus; IC:MGI.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; IDA:MGI.
 GO:0007050; P:cell cycle arrest; IDA:MGI.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006464; P:cellular protein modification process; IEA:InterPro.
 GO:0030216; P:keratinocyte differentiation; IDA:MGI.
 GO:0008285; P:negative regulation of cell proliferation; IDA:MGI.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:MGI.
 GO:0046755; P:non-lytic virus budding; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0001558; P:regulation of cell growth; IDA:MGI.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISS:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR017916; Steadiness_box.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR008883; UEV_N. 
Pfam
 PF05743; UEV
 PF09454; Vps23_core 
SMART
  
PROSITE
 PS51312; SB
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2
 PS51322; UEV 
PRINTS