UniProt Accession

 MPPE1_HUMAN; Q53F39; B0YJ39; B0YJ40; B0YJ41; B5ME53; B7WNJ3; D3DUI5; D3DUI7; Q6GMP1; Q8TAD6; Q8TE26; Q8WZ32; Q9BU58; Q9H958; Q9HAI4 

Genbank Protein ID

 AF363483; AF363482; AF363483; AF363482; AF363484; AK021647; AK023052; AK223450; AF289560; EF444996; EF444996; EF444996; AP001269; CH471113; CH471113; CH471113; CH471113; CH471113; CH471113; CH471113; BC002877; BC073994 

Genbank Nucleotide ID

 AAM00277.1; AAM00277.1; AAM00278.1; AAM00278.1; AAM00279.1; BAB13863.1; BAB14378.1; BAD97170.1; AAL55744.1; ACA06017.1; ACA06018.1; ACA06019.1; EAX01562.1; EAX01565.1; EAX01564.1; EAX01569.1; EAX01571.1; EAX01567.1; EAX01568.1; AAH02877.1; AAH73994.1 

Protein Name

 Metallophosphoesterase 1 

Protein Synonyms/Alias

 Post-GPI attachment to proteins factor 5 

Gene Name

 MPPE1 

Gene Synonyms/Alias

 PGAP5; PP579 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
176	YKVERFEKVFSSERL	ubiquitination	[1]
276	EERDIPFKENYDVLS	ubiquitination	[1]
289	LSREASQKLLWWLQP	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Metallophosphoesterase required for transport of GPI- anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins. 

Sequence Annotation

 MOTIF 392 396 Di-lysine motif.
 METAL 77 77 Divalent metal cation 2 (Probable).
 METAL 119 119 Divalent metal cation 1 (Probable).
 METAL 119 119 Divalent metal cation 2 (Probable).
 METAL 157 157 Divalent metal cation 1 (Probable).
 METAL 249 249 Divalent metal cation 1 (Probable).
 METAL 303 303 Divalent metal cation 1 (Probable).
 METAL 305 305 Divalent metal cation 2 (Probable).  

Keyword

 Alternative splicing; Complete proteome; ER-Golgi transport; Golgi apparatus; GPI-anchor biosynthesis; Hydrolase; Manganese; Membrane; Metal-binding; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 396 AA 

Protein Sequence

Gene Ontology

 GO:0005801; C:cis-Golgi network; ISS:UniProtKB.
 GO:0070971; C:endoplasmic reticulum exit site; ISS:UniProtKB.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; ISS:UniProtKB.
 GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0034235; F:GPI anchor binding; ISS:UniProtKB.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0008081; F:phosphoric diester hydrolase activity; ISS:UniProtKB.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; ISS:UniProtKB.
 GO:0006506; P:GPI anchor biosynthetic process; ISS:UniProtKB. 

Interpro

 IPR024654; Calcineurin-like_PEstase_dom. 

Pfam

 PF12850; Metallophos_2 

SMART

  

PROSITE

  

PRINTS