CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012467
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable E3 ubiquitin-protein ligase HERC3 
Protein Synonyms/Alias
 HECT domain and RCC1-like domain-containing protein 3 
Gene Name
 HERC3 
Gene Synonyms/Alias
 KIAA0032 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37FISDRSVKEVACGGNubiquitination[1]
63YTCGLNTKGQLGHERubiquitination[1]
74GHEREGNKPEQIGALubiquitination[1]
173HGQLGLGKEFPSQASubiquitination[1, 2, 3]
237RESPCHVKLLRTQKVubiquitination[1]
336TGHTCNVKCPSPVKGubiquitination[1]
342VKCPSPVKGYWAAHSubiquitination[1]
450KKIDEHFKTSPKIPGubiquitination[1]
821YKKLLNVKPGLEDLKubiquitination[1, 3]
871YGVIEQKKLIPGGDNubiquitination[1]
920FLKVCGGKVLELFQPubiquitination[1]
952LEETAIYKGDYSATHubiquitination[3]
976FHEFPLEKKKKFLLFubiquitination[1]
1030DLPKYSSKEILSARLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). 
Sequence Annotation
 REPEAT 1 51 RCC1 1.
 REPEAT 52 101 RCC1 2.
 REPEAT 102 154 RCC1 3.
 REPEAT 156 207 RCC1 4.
 REPEAT 208 259 RCC1 5.
 REPEAT 261 311 RCC1 6.
 REPEAT 313 366 RCC1 7.
 DOMAIN 951 1050 HECT.
 ACT_SITE 1018 1018 Glycyl thioester intermediate (By  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; Ligase; Polymorphism; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1050 AA 
Protein Sequence
MLCWGYWSLG QPGISTNLQG IVAEPQVCGF ISDRSVKEVA CGGNHSVFLL EDGEVYTCGL 60
NTKGQLGHER EGNKPEQIGA LADQHIIHVA CGESHSLALS DRGQLFSWGA GSDGQLGLMT 120
TEDSVAVPRL IQKLNQQTIL QVSCGNWHCL ALAADGQFFT WGKNSHGQLG LGKEFPSQAS 180
PQRVRSLEGI PLAQVAAGGA HSFALSLSGA VFGWGMNNAG QLGLSDEKDR ESPCHVKLLR 240
TQKVVYISCG EEHTAVLTKS GGVFTFGAGS CGQLGHDSMN DEVNPRRVLE LMGSEVTQIA 300
CGRQHTLAFV PSSGLIYAFG CGARGQLGTG HTCNVKCPSP VKGYWAAHSG QLSARADRFK 360
YHIVKQIFSG GDQTFVLCSK YENYSPAVDF RTMNQAHYTS LINDETIAVW RQKLSEHNNA 420
NTINGVVQIL SSAACWNGSF LEKKIDEHFK TSPKIPGIDL NSTRVLFEKL MNSQHSMILE 480
QILNSFESCL IPQLSSSPPD VEAMRIYLIL PEFPLLQDSK YYITLTIPLA MAILRLDTNP 540
SKVLDNWWSQ VCPKYFMKLV NLYKGAVLYL LRGRKTFLIP VLFNNYITAA LKLLEKLYKV 600
NLKVKHVEYD TFYIPEISNL VDIQEDYLMW FLHQAGMKAR PSIIQDTVTL CSYPFIFDAQ 660
AKTKMLQTDA ELQMQVAVNG ANLQNVFMLL TLEPLLARSP FLVLHVRRNN LVGDALRELS 720
IHSDIDLKKP LKVIFDGEEA VDAGGVTKEF FLLLLKELLN PIYGMFTYYQ DSNLLWFSDT 780
CFVEHNWFHL IGITCGLAIY NSTVVDLHFP LALYKKLLNV KPGLEDLKEL SPTEGRSLQE 840
LLDYPGEDVE ETFCLNFTIC RESYGVIEQK KLIPGGDNVT VCKDNRQEFV DAYVNYVFQI 900
SVHEWYTAFS SGFLKVCGGK VLELFQPSEL RAMMVGNSNY NWEELEETAI YKGDYSATHP 960
TVKLFWETFH EFPLEKKKKF LLFLTGSDRI PIYGMASLQI VIQSTASGEE YLPVAHTCYN 1020
LLDLPKYSSK EILSARLTQA LDNYEGFSLA 1050 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0004842; F:ubiquitin-protein ligase activity; IBA:RefGenome.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. 
Interpro
 IPR000569; HECT.
 IPR009091; RCC1/BLIP-II.
 IPR000408; Reg_chr_condens. 
Pfam
 PF00632; HECT
 PF00415; RCC1 
SMART
 SM00119; HECTc 
PROSITE
 PS50237; HECT
 PS00625; RCC1_1
 PS00626; RCC1_2
 PS50012; RCC1_3 
PRINTS
 PR00633; RCCNDNSATION.