CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021386
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 EH domain-containing protein 1 
Protein Synonyms/Alias
 PAST homolog 1; hPAST1; Testilin 
Gene Name
 EHD1 
Gene Synonyms/Alias
 PAST; PAST1; CDABP0131 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MFSWVSKDARRKKEubiquitination[1]
32LRQLYAQKLLPLEEHubiquitination[1, 2]
124DPRRPFRKLNAFGNAubiquitination[1]
220KIRVVLNKADQIETQubiquitination[1, 3]
280AEEQDLFKDIQSLPRubiquitination[3]
328VFGKESKKKELVNNLubiquitination[1]
329FGKESKKKELVNNLGubiquitination[1]
341NLGEIYQKIEREHQIubiquitination[1]
475KKEMVKSKLPNTVLGubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. 
Sequence Annotation
 DOMAIN 444 532 EH.
 DOMAIN 476 511 EF-hand.
 NP_BIND 65 72 ATP (By similarity).
 BINDING 220 220 ATP (By similarity).
 BINDING 258 258 ATP (By similarity).
 MOD_RES 456 456 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Calcium; Cell membrane; Coiled coil; Complete proteome; Direct protein sequencing; Endosome; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 534 AA 
Protein Sequence
MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL EDADFDNKPM 60
VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV MHGPTEGVVP GNALVVDPRR 120
PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS IIDTPGILSG EKQRISRGYD FAAVLEWFAE 180
RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL 240
GKIINTPEVV RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR 300
ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISP GDFPSLRKMQ 360
ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE ESLMPSQVVK GGAFDGTMNG 420
PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE IFYTLSPVNG KITGANAKKE MVKSKLPNTV 480
LGKIWKLADV DKDGLLDDEE FALANHLIKV KLEGHELPAD LPPHLVPPSK RRHE 534 
Gene Ontology
 GO:0031901; C:early endosome membrane; IDA:UniProtKB.
 GO:0030139; C:endocytic vesicle; IEA:Compara.
 GO:0005811; C:lipid particle; ISS:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0031095; C:platelet dense tubular network membrane; ISS:BHF-UCL.
 GO:0055038; C:recycling endosome membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0042632; P:cholesterol homeostasis; ISS:BHF-UCL.
 GO:0032456; P:endocytic recycling; IMP:UniProtKB.
 GO:0006897; P:endocytosis; IEA:Compara.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0006886; P:intracellular protein transport; IMP:UniProtKB.
 GO:0034383; P:low-density lipoprotein particle clearance; ISS:BHF-UCL.
 GO:0010886; P:positive regulation of cholesterol storage; ISS:BHF-UCL.
 GO:2001137; P:positive regulation of endocytic recycling; ISS:UniProtKB.
 GO:1901741; P:positive regulation of myoblast fusion; ISS:UniProtKB.
 GO:0051260; P:protein homooligomerization; IPI:UniProtKB. 
Interpro
 IPR001401; Dynamin_GTPase.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N 
SMART
 SM00027; EH 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS