CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010952
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein MLP1 
Protein Synonyms/Alias
 Myosin-like protein 1 
Gene Name
 MLP1 
Gene Synonyms/Alias
 YKR095W; YKR415 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
146SNQQRTLKILDERLKacetylation[1]
359EKLAQCEKECLRLSSubiquitination[2]
462ELNAKNQKLVECENDubiquitination[2]
475NDLQTLTKQRLDLCRubiquitination[2]
551EKNAELLKVVRNLADubiquitination[2]
573KSKQSLQKIESETVNacetylation[1]
593IITLKSEKMDLESRIubiquitination[2]
672IQDLYDSKSDISIKLubiquitination[2]
820EDALSELKKETSQKDacetylation[1]
968EKTSLEDKISLLKEQacetylation[1]
1751KRESLANKTDPPTHLacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Involved together with the closely related MLP2 in the structural and functional organization of perinuclear chromatin. MLP1/MLP2 associate with the nuclear pore complex and form filamentous structures along the nuclear periphery. According to PubMed:11862215 some telomeres are tethered to the nuclear periphery through MLP1/MLP2, probably mediated by YKU70/YKU80 (HDF1/HDF2) heterodimer and show perinuclear location dependent silencing. According to PubMed:12490156 MLP1 and MLP2 are involved in telomere length regulation but not silencing or telomere anchoring. MLP1 also recognizes the 5'-splice site of pre-mRNAs and retains unspliced pre-mRNA in the nucleus without affecting splicing itself. 
Sequence Annotation
 MOTIF 1496 1565 Required for nuclear localization.
 MOD_RES 337 337 Phosphothreonine.
 MOD_RES 379 379 Phosphoserine.
 MOD_RES 1670 1670 Phosphoserine.
 MOD_RES 1710 1710 Phosphoserine.
 MOD_RES 1733 1733 Phosphoserine.
 MOD_RES 1803 1803 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; DNA damage; DNA repair; mRNA transport; Nucleus; Phosphoprotein; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1875 AA 
Protein Sequence
MSDHDTPMES IQNGENSDER LNAIASFFGC SLEQVKSFDG DVVKHLNDKL LQFNELKSEN 60
LKVTVSFDEL KASSLKKIDG LKTEMENVIR ENDKIRKERN DTFVKFESVE NEKMKLSSEL 120
EFVKRKLDDL TEEKKETQSN QQRTLKILDE RLKEIELVRV ENNRSNSECK KLRSTIMDLE 180
TKQQGYITND LNSRTELERK TQELTLLQSN NDWLEKELRS KNEQYLSYRQ KTDKVILDIR 240
NELNRLRNDF QMERTNNDVL KQKNNELSKS LQEKLLEIKG LSDSLNSEKQ EFSAEMSLKQ 300
RLVDLLESQL NAVKEELNSI RELNTAKVIA DDSKKQTPEN EDLLKELQLT KEKLAQCEKE 360
CLRLSSITDE ADEDNENLSA KSSSDFIFLK KQLIKERRTK EHLQNQIETF IVELEHKVPI 420
INSFKERTDM LENELNNAAL LLEHTSNEKN AKVKELNAKN QKLVECENDL QTLTKQRLDL 480
CRQIQYLLIT NSVSNDSKGP LRKEEIQFIQ NIMQEDDSTI TESDSQKVVT ERLVEFKNII 540
QLQEKNAELL KVVRNLADKL ESKEKKSKQS LQKIESETVN EAKEAIITLK SEKMDLESRI 600
EELQKELEEL KTSVPNEDAS YSNVTIKQLT ETKRDLESQV QDLQTRISQI TRESTENMSL 660
LNKEIQDLYD SKSDISIKLG KEKSSRILAE ERFKLLSNTL DLTKAENDQL RKRFDYLQNT 720
ILKQDSKTHE TLNEYVSCKS KLSIVETELL NLKEEQKLRV HLEKNLKQEL NKLSPEKDSL 780
RIMVTQLQTL QKEREDLLEE TRKSCQKKID ELEDALSELK KETSQKDHHI KQLEEDNNSN 840
IEWYQNKIEA LKKDYESVIT SVDSKQTDIE KLQYKVKSLE KEIEEDKIRL HTYNVMDETI 900
NDDSLRKELE KSKINLTDAY SQIKEYKDLY ETTSQSLQQT NSKLDESFKD FTNQIKNLTD 960
EKTSLEDKIS LLKEQMFNLN NELDLQKKGM EKEKADFKKR ISILQNNNKE VEAVKSEYES 1020
KLSKIQNDLD QQTIYANTAQ NNYEQELQKH ADVSKTISEL REQLHTYKGQ VKTLNLSRDQ 1080
LENALKENEK SWSSQKESLL EQLDLSNSRI EDLSSQNKLL YDQIQIYTAA DKEVNNSTNG 1140
PGLNNILITL RRERDILDTK VTVAERDAKM LRQKISLMDV ELQDARTKLD NSRVEKENHS 1200
SIIQQHDDIM EKLNQLNLLR ESNITLRNEL ENNNNKKKEL QSELDKLKQN VAPIESELTA 1260
LKYSMQEKEQ ELKLAKEEVH RWKKRSQDIL EKHEQLSSSD YEKLESEIEN LKEELENKER 1320
QGAEAEEKFN RLRRQAQERL KTSKLSQDSL TEQVNSLRDA KNVLENSLSE ANARIEELQN 1380
AKVAQGNNQL EAIRKLQEDA EKASRELQAK LEESTTSYES TINGLNEEIT TLKEEIEKQR 1440
QIQQQLQATS ANEQNDLSNI VESMKKSFEE DKIKFIKEKT QEVNEKILEA QERLNQPSNI 1500
NMEEIKKKWE SEHEQEVSQK IREAEEALKK RIRLPTEEKI NKIIERKKEE LEKEFEEKVE 1560
ERIKSMEQSG EIDVVLRKQL EAKVQEKQKE LENEYNKKLQ EELKDVPHSS HISDDERDKL 1620
RAEIESRLRE EFNNELQAIK KKSFDEGKQQ AMMKTTLLER KLAKMESQLS ETKQSAESPP 1680
KSVNNVQNPL LGLPRKIEEN SNSPFNPLLS GEKLLKLNSK SSSGGFNPFT SPSPNKHLQN 1740
DNDKRESLAN KTDPPTHLEP SFNIPASRGL ISSSSTLSTD TNDEELTSNN PAQKDSSNRN 1800
VQSEEDTEKK KEGEPVKRGE AIEEQTKSNK RPIDEVGELK NDEDDTTENI NESKKIKTED 1860
EEEKETDKVN DENSI 1875 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0005643; C:nuclear pore; IEA:InterPro.
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0043021; F:ribonucleoprotein complex binding; IPI:SGD.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:1901925; P:negative regulation of protein import into nucleus during spindle assembly checkpoint; IGI:SGD.
 GO:0071048; P:nuclear retention of unspliced pre-mRNA at the site of transcription; IMP:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
 GO:0006606; P:protein import into nucleus; IGI:SGD.
 GO:0090204; P:protein localization to nuclear pore; IMP:SGD.
 GO:0034398; P:telomere tethering at nuclear periphery; IGI:SGD.
 GO:0090203; P:transcriptional activation by promoter-terminator looping; IMP:SGD. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR012929; TPR_MLP1_2. 
Pfam
 PF07926; TPR_MLP1_2 
SMART
  
PROSITE
 PS51450; LRR 
PRINTS