CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000698
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U4/U6 small nuclear ribonucleoprotein Prp3 
Protein Synonyms/Alias
 Pre-mRNA-splicing factor 3; hPrp3; U4/U6 snRNP 90 kDa protein 
Gene Name
 PRPF3 
Gene Synonyms/Alias
 HPRP3; PRP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
52KKAADHLKPFLDDSTubiquitination[1, 2]
298EQFKQQLKEKPSEDMubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. 
Sequence Annotation
 DOMAIN 1 87 PWI.
 MOD_RES 164 164 Phosphoserine.
 MOD_RES 167 167 Phosphothreonine.
 MOD_RES 619 619 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Disease mutation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Retinitis pigmentosa; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 683 AA 
Protein Sequence
MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH LKPFLDDSTL 60
RFVDKLFEAV EEGRSSRHSK SSSDRSRKRE LKEVFGDDSE ISKESSGVKK RRIPRFEEVE 120
EEPEVIPGPP SESPGMLTKL QIKQMMEAAT RQIEERKKQL SFISPPTPQP KTPSSSQPER 180
LPIGNTIQPS QAATFMNDAI EKARKAAELQ ARIQAQLALK PGLIGNANMV GLANLHAMGI 240
APPKVELKDQ TKPTPLILDE QGRTVDATGK EIELTHRMPT LKANIRAVKR EQFKQQLKEK 300
PSEDMESNTF FDPRVSIAPS QRQRRTFKFH DKGKFEKIAQ RLRTKAQLEK LQAEISQAAR 360
KTGIHTSTRL ALIAPKKELK EGDIPEIEWW DSYIIPNGFD LTEENPKRED YFGITNLVEH 420
PAQLNPPVDN DTPVTLGVYL TKKEQKKLRR QTRREAQKEL QEKVRLGLMP PPEPKVRISN 480
LMRVLGTEAV QDPTKVEAHV RAQMAKRQKA HEEANAARKL TAEQRKVKKI KKLKEDISQG 540
VHISVYRVRN LSNPAKKFKI EANAGQLYLT GVVVLHKDVN VVVVEGGPKA QKKFKRLMLH 600
RIKWDEQTSN TKGDDDEESD EEAVKKTNKC VLVWEGTAKD RSFGEMKFKQ CPTENMAREH 660
FKKHGAEHYW DLALSESVLE STD 683 
Gene Ontology
 GO:0015030; C:Cajal body; IDA:BHF-UCL.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; NAS:UniProtKB.
 GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IEA:InterPro.
 GO:0000398; P:mRNA splicing, via spliceosome; NAS:UniProtKB. 
Interpro
 IPR010541; DUF1115.
 IPR013881; Pre-mRNA_splic_Prp3.
 IPR027104; Prp3.
 IPR002483; PWI_dom. 
Pfam
 PF06544; DUF1115
 PF08572; PRP3
 PF01480; PWI 
SMART
 SM00311; PWI 
PROSITE
 PS51025; PWI 
PRINTS