UniProt Accession

 ARK72_HUMAN; O43488; O75749; Q5TG63 

Genbank Protein ID

 AL035413; BC004111; BC007352; BC010852; BC011586; BC012171; BC013996; AF026947; Y16675; BT007347; BK000395 

Genbank Nucleotide ID

 CAB72321.1; AAH04111.3; AAH07352.2; AAH10852.1; AAH11586.1; AAH12171.1; AAH13996.1; AAC52104.1; CAA76347.1; AAP36011.1; DAA00088.1 

Protein Name

 Aflatoxin B1 aldehyde reductase member 2 

Protein Synonyms/Alias

 AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase 

Gene Name

 AKR7A2 

Gene Synonyms/Alias

 AFAR; AFAR1; AKR7 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
105	CRVKIATKANPWDGK	ubiquitination	[1]
112	KANPWDGKSLKPDSV	ubiquitination	[1]
115	PWDGKSLKPDSVRSQ	ubiquitination	[1]
128	SQLETSLKRLQCPQV	ubiquitination	[1]
236	AGGLLTGKYKYEDKD	ubiquitination	[1, 2, 3, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2- nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. 

Sequence Annotation

 NP_BIND 171 172 NADP.
 NP_BIND 226 236 NADP.
 NP_BIND 318 326 NADP.
 ACT_SITE 77 77 Proton donor (By similarity).
 BINDING 72 72 NADP.
 BINDING 141 141 Substrate (By similarity).
 BINDING 197 197 NADP.
 BINDING 250 250 NADP.
 BINDING 260 260 Substrate (By similarity).
 BINDING 263 263 Substrate (By similarity).
 BINDING 359 359 Substrate (By similarity).
 MOD_RES 40 40 Phosphoserine (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Golgi apparatus; NADP; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 359 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; TAS:ProtInc.
 GO:0009055; F:electron carrier activity; TAS:UniProtKB.
 GO:0019119; F:phenanthrene-9,10-epoxide hydrolase activity; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; TAS:ProtInc.
 GO:0006081; P:cellular aldehyde metabolic process; TAS:ProtInc.
 GO:0044597; P:daunorubicin metabolic process; IMP:UniProtKB.
 GO:0044598; P:doxorubicin metabolic process; IMP:UniProtKB. 

Interpro

 IPR001395; Aldo/ket_red.
 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

  

PRINTS