UniProt Accession

 CELF4_MOUSE; Q7TSY6; Q80Y76; Q811Z7; Q8BKM2; Q8BLM9; Q8BXA2; Q99PE0 

Genbank Protein ID

 AF515450; AK044072; AK048427; AK051458; AC121094; AC144938; AC153142; BC048405; BC052744; AF314173 

Genbank Nucleotide ID

 AAO22167.1; BAC31762.1; BAC33334.1; BAC34649.1; AAH48405.1; AAH52744.1; AAK00298.1 

Protein Name

 CUGBP Elav-like family member 4 

Protein Synonyms/Alias

 CELF-4; Bruno-like protein 4; CUG-BP- and ETR-3-like factor 4; RNA-binding protein BRUNOL-4 

Gene Name

 Celf4 

Gene Synonyms/Alias

 Brul4; Brunol4 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
68	IPRNLDEKDLKPLFE	ubiquitination	[1]
71	NLDEKDLKPLFEEFG	ubiquitination	[1]
87	IYELTVLKDRFTGMH	ubiquitination	[1]
153	QPPSQDRKLFVGMLN	ubiquitination	[1]
161	LFVGMLNKQQSEDDV	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 RNA-binding protein implicated in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity). 

Sequence Annotation

 DOMAIN 54 135 RRM 1.
 DOMAIN 152 232 RRM 2.
 DOMAIN 406 479 RRM 3.
 REGION 1 298 Sufficient for RNA-binding and MSE-
 REGION 239 258 Necessary for TNNT2 exon 5 inclusion (By  

Keyword

 Activator; Alternative splicing; Complete proteome; Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 486 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:MGI.
 GO:0042835; F:BRE binding; ISS:UniProtKB.
 GO:0003729; F:mRNA binding; IDA:MGI.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0000380; P:alternative mRNA splicing, via spliceosome; IEA:Compara.
 GO:0006376; P:mRNA splice site selection; IDA:MGI.
 GO:0090394; P:negative regulation of excitatory postsynaptic membrane potential; IMP:MGI.
 GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
 GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IEA:Compara. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS