UniProt Accession

 METK1_MOUSE; Q91X83 

Genbank Protein ID

 AK149542; AK153832; BC011211 

Genbank Nucleotide ID

 BAE28947.1; BAE32202.1; AAH11211.1 

Protein Name

 S-adenosylmethionine synthase isoform type-1 

Protein Synonyms/Alias

 AdoMet synthase 1; Methionine adenosyltransferase 1; MAT 1 

Gene Name

 Mat1a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
48	AVLDAHLKQDPNAKV	ubiquitination	[1]
54	LKQDPNAKVACETVC	ubiquitination	[1]
89	RVVRDTIKHIGYDDS	acetylation	[2]
89	RVVRDTIKHIGYDDS	ubiquitination	[1]
98	IGYDDSAKGFDFKTC	ubiquitination	[1]
160	LTIVLAHKLNTRIAD	ubiquitination	[1]
229	ALKEQVIKAVVPAKY	ubiquitination	[1]
235	IKAVVPAKYLDEDTV	acetylation	[2, 3, 4]
235	IKAVVPAKYLDEDTV	ubiquitination	[1]
266	DAGVTGRKIIVDTYG	ubiquitination	[1]
286	GGGAFSGKDYTKVDR	ubiquitination	[1]
304	YAARWVAKSLVKAGL	ubiquitination	[1]
308	WVAKSLVKAGLCRRV	ubiquitination	[1]
352	ELLEVVNKNFDLRPG	ubiquitination	[1]
368	IVRDLDLKKPIYQKT	acetylation	[2]
368	IVRDLDLKKPIYQKT	ubiquitination	[1]
369	VRDLDLKKPIYQKTA	acetylation	[2]
369	VRDLDLKKPIYQKTA	ubiquitination	[1]
374	LKKPIYQKTACYGHF	ubiquitination	[1]
392	EFPWEVPKKLVF***	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Catalyzes the formation of S-adenosylmethionine from methionine and ATP (By similarity). 

Sequence Annotation

 NP_BIND 132 137 ATP (Potential).
 METAL 32 32 Magnesium (By similarity).
 METAL 58 58 Potassium (By similarity).
 METAL 284 284 Potassium (By similarity).
 METAL 292 292 Magnesium (By similarity).
 BINDING 30 30 Substrate (By similarity).
 BINDING 160 160 ATP (Potential).
 BINDING 180 180 Substrate (By similarity).
 BINDING 248 248 Substrate (By similarity).
 BINDING 250 250 Substrate; via carbonyl oxygen (By
 BINDING 259 259 Substrate (By similarity).
 MOD_RES 121 121 S-nitrosocysteine (By similarity).
 DISULFID 35 61 By similarity.  

Keyword

 ATP-binding; Cobalt; Complete proteome; Disulfide bond; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; S-nitrosylation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 396 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004478; F:methionine adenosyltransferase activity; TAS:MGI.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 

Pfam

 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 

SMART

  

PROSITE

 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 

PRINTS