CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012604
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mediator of RNA polymerase II transcription subunit 1 
Protein Synonyms/Alias
 Activator-recruited cofactor 205 kDa component; ARC205; Mediator complex subunit 1; Peroxisome proliferator-activated receptor-binding protein; PBP; PPAR-binding protein; Thyroid hormone receptor-associated protein complex 220 kDa component; Trap220; Thyroid receptor-interacting protein 2; TR-interacting protein 2; TRIP-2; Vitamin D receptor-interacting protein complex component DRIP205; p53 regulatory protein RB18A 
Gene Name
 MED1 
Gene Synonyms/Alias
 ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15EESEKLSKMSSLLERacetylation[1]
26LLERLHAKFNQNRPWubiquitination[2]
202TNAGPLDKILHGSVGubiquitination[2]
235PSDLLDDKTASPIILubiquitination[2]
378QHCYFLNKDAPLPDGubiquitination[2]
426TLIGSCVKRTILKEDubiquitination[2]
431CVKRTILKEDSPGLLubiquitination[2]
481HVSCKLYKGLSDALIubiquitination[2]
496CTDDFIAKVVQRCMSubiquitination[2]
991SGPGLDSKPGKRSRTacetylation[3]
1177TKMKPQGKPSSLMNPacetylation[1]
1210DKLASPMKPVPGTPPacetylation[3]
1309SLTAVIDKLKHGVVTacetylation[1]
1354SGGEFQGKREKSDKDacetylation[1, 4]
1529KKKSHSIKPESWSKSacetylation[1, 5]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 
Functional Description
 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
Sequence Annotation
 REGION 1 670 Interaction with the Mediator complex and
 REGION 16 590 Interaction with ESR1.
 REGION 108 212 Interaction with the Mediator complex.
 REGION 215 390 Interaction with the Mediator complex.
 REGION 405 644 Interaction with THRA.
 REGION 542 789 Interaction with VDR.
 REGION 622 701 Interaction with PPARGC1A and THRA.
 REGION 637 716 Interaction with GATA1 (By similarity).
 REGION 656 1066 Interaction with ESR1.
 REGION 1249 1421 Interaction with TP53.
 MOTIF 604 608 LXXLL motif 1.
 MOTIF 645 649 LXXLL motif 2.
 MOD_RES 664 664 Phosphoserine.
 MOD_RES 795 795 Phosphoserine.
 MOD_RES 805 805 Phosphothreonine.
 MOD_RES 953 953 Phosphoserine (By similarity).
 MOD_RES 1032 1032 Phosphothreonine; by MAPK1 or MAPK3.
 MOD_RES 1051 1051 Phosphothreonine.
 MOD_RES 1057 1057 Phosphothreonine.
 MOD_RES 1156 1156 Phosphoserine.
 MOD_RES 1177 1177 N6-acetyllysine.
 MOD_RES 1207 1207 Phosphoserine.
 MOD_RES 1215 1215 Phosphothreonine.
 MOD_RES 1223 1223 Phosphoserine.
 MOD_RES 1347 1347 Phosphoserine.
 MOD_RES 1403 1403 Phosphoserine.
 MOD_RES 1433 1433 Phosphoserine.
 MOD_RES 1457 1457 Phosphothreonine; by MAPK1 or MAPK3.
 MOD_RES 1463 1463 Phosphoserine.
 MOD_RES 1479 1479 Phosphoserine.
 MOD_RES 1481 1481 Phosphoserine.
 MOD_RES 1482 1482 Phosphoserine.
 MOD_RES 1529 1529 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1581 AA 
Protein Sequence
MKAQGETEES EKLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS 60
CLETLQKALK VTSLPAMTDR LESIARQNGL GSHLSASGTE CYITSDMFYV EVQLDPAGQL 120
CDVKVAHHGE NPVSCPELVQ QLREKNFDEF SKHLKGLVNL YNLPGDNKLK TKMYLALQSL 180
EQDLSKMAIM YWKATNAGPL DKILHGSVGY LTPRSGGHLM NLKYYVSPSD LLDDKTASPI 240
ILHENNVSRS LGMNASVTIE GTSAVYKLPI APLIMGSHPV DNKWTPSFSS ITSANSVDLP 300
ACFFLKFPQP IPVSRAFVQK LQNCTGIPLF ETQPTYAPLY ELITQFELSK DPDPIPLNHN 360
MRFYAALPGQ QHCYFLNKDA PLPDGRSLQG TLVSKITFQH PGRVPLILNL IRHQVAYNTL 420
IGSCVKRTIL KEDSPGLLQF EVCPLSESRF SVSFQHPVND SLVCVVMDVQ DSTHVSCKLY 480
KGLSDALICT DDFIAKVVQR CMSIPVTMRA IRRKAETIQA DTPALSLIAE TVEDMVKKNL 540
PPASSPGYGM TTGNNPMSGT TTPTNTFPGG PITTLFNMSM SIKDRHESVG HGEDFSKVSQ 600
NPILTSLLQI TGNGGSTIGS SPTPPHHTPP PVSSMAGNTK NHPMLMNLLK DNPAQDFSTL 660
YGSSPLERQN SSSGSPRMEI CSGSNKTKKK KSSRLPPEKP KHQTEDDFQR ELFSMDVDSQ 720
NPIFDVNMTA DTLDTPHITP APSQCSTPPT TYPQPVPHPQ PSIQRMVRLS SSDSIGPDVT 780
DILSDIAEEA SKLPSTSDDC PAIGTPLRDS SSSGHSQSTL FDSDVFQTNN NENPYTDPAD 840
LIADAAGSPS SDSPTNHFFH DGVDFNPDLL NSQSQSGFGE EYFDESSQSG DNDDFKGFAS 900
QALNTLGVPM LGGDNGETKF KGNNQADTVD FSIISVAGKA LAPADLMEHH SGSQGPLLTT 960
GDLGKEKTQK RVKEGNGTSN STLSGPGLDS KPGKRSRTPS NDGKSKDKPP KRKKADTEGK 1020
SPSHSSSNRP FTPPTSTGGS KSPGSAGRSQ TPPGVATPPI PKITIQIPKG TVMVGKPSSH 1080
SQYTSSGSVS SSGSKSHHSH SSSSSSSAST SGKMKSSKSE GSSSSKLSSS MYSSQGSSGS 1140
SQSKNSSQSG GKPGSSPITK HGLSSGSSST KMKPQGKPSS LMNPSLSKPN ISPSHSRPPG 1200
GSDKLASPMK PVPGTPPSSK AKSPISSGSG GSHMSGTSSS SGMKSSSGLG SSGSLSQKTP 1260
PSSNSCTASS SSFSSSGSSM SSSQNQHGSS KGKSPSRNKK PSLTAVIDKL KHGVVTSGPG 1320
GEDPLDGQMG VSTNSSSHPM SSKHNMSGGE FQGKREKSDK DKSKVSTSGS SVDSSKKTSE 1380
SKNVGSTGVA KIIISKHDGG SPSIKAKVTL QKPGESSGEG LRPQMASSKN YGSPLISGST 1440
PKHERGSPSH SKSPAYTPQN LDSESESGSS IAEKSYQNSP SSDDGIRPLP EYSTEKHKKH 1500
KKEKKKVKDK DRDRDRDKDR DKKKSHSIKP ESWSKSPISS DQSLSMTSNT ILSADRPSRL 1560
SPDFMIGEED DDLMDVALIG N 1581 
Gene Ontology
 GO:0016592; C:mediator complex; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IMP:UniProtKB.
 GO:0001047; F:core promoter binding; IDA:UniProtKB.
 GO:0016922; F:ligand-dependent nuclear receptor binding; IDA:UniProtKB.
 GO:0036033; F:mediator complex binding; IDA:UniProtKB.
 GO:0004872; F:receptor activity; IDA:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IDA:UniProtKB.
 GO:0000981; F:sequence-specific DNA binding RNA polymerase II transcription factor activity; ISS:UniProtKB.
 GO:0046966; F:thyroid hormone receptor binding; IDA:UniProtKB.
 GO:0030375; F:thyroid hormone receptor coactivator activity; IMP:UniProtKB.
 GO:0042809; F:vitamin D receptor binding; NAS:UniProtKB.
 GO:0006702; P:androgen biosynthetic process; IMP:UniProtKB.
 GO:0030521; P:androgen receptor signaling pathway; IDA:UniProtKB.
 GO:0001525; P:angiogenesis; ISS:UniProtKB.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0000902; P:cell morphogenesis; IMP:UniProtKB.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0071364; P:cellular response to epidermal growth factor stimulus; IDA:UniProtKB.
 GO:0035729; P:cellular response to hepatocyte growth factor stimulus; IEA:Compara.
 GO:0071383; P:cellular response to steroid hormone stimulus; IDA:UniProtKB.
 GO:0097067; P:cellular response to thyroid hormone stimulus; IDA:UniProtKB.
 GO:0006260; P:DNA replication; IEA:Compara.
 GO:0035050; P:embryonic heart tube development; IEA:Compara.
 GO:0035162; P:embryonic hemopoiesis; IEA:Compara.
 GO:0035116; P:embryonic hindlimb morphogenesis; IEA:Compara.
 GO:0001892; P:embryonic placenta development; IEA:Compara.
 GO:0048822; P:enucleate erythrocyte development; IEA:Compara.
 GO:0060750; P:epithelial cell proliferation involved in mammary gland duct elongation; IEA:Compara.
 GO:0070371; P:ERK1 and ERK2 cascade; IDA:UniProtKB.
 GO:0048821; P:erythrocyte development; ISS:UniProtKB.
 GO:0045444; P:fat cell differentiation; IDA:MGI.
 GO:0030216; P:keratinocyte differentiation; IMP:UniProtKB.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0002088; P:lens development in camera-type eye; ISS:UniProtKB.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0060745; P:mammary gland branching involved in pregnancy; IEA:Compara.
 GO:0060744; P:mammary gland branching involved in thelarche; IEA:Compara.
 GO:0035855; P:megakaryocyte development; ISS:UniProtKB.
 GO:0030224; P:monocyte differentiation; IEA:Compara.
 GO:0042789; P:mRNA transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0010839; P:negative regulation of keratinocyte proliferation; IMP:UniProtKB.
 GO:0045665; P:negative regulation of neuron differentiation; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0035357; P:peroxisome proliferator activated receptor signaling pathway; IEA:Compara.
 GO:0070318; P:positive regulation of G0 to G1 transition; IEA:Compara.
 GO:2000347; P:positive regulation of hepatocyte proliferation; IEA:Compara.
 GO:0060335; P:positive regulation of interferon-gamma-mediated signaling pathway; IEA:Compara.
 GO:0033148; P:positive regulation of intracellular estrogen receptor signaling pathway; IEA:Compara.
 GO:0045618; P:positive regulation of keratinocyte differentiation; IMP:UniProtKB.
 GO:0033601; P:positive regulation of mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0033160; P:positive regulation of protein import into nucleus, translocation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0051726; P:regulation of cell cycle; NAS:UniProtKB.
 GO:0006356; P:regulation of transcription from RNA polymerase I promoter; IDA:UniProtKB.
 GO:0070562; P:regulation of vitamin D receptor signaling pathway; IEA:Compara.
 GO:0003406; P:retinal pigment epithelium development; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006590; P:thyroid hormone generation; IEA:Compara.
 GO:0002154; P:thyroid hormone mediated signaling pathway; IMP:UniProtKB.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0003222; P:ventricular trabecula myocardium morphogenesis; IEA:Compara. 
Interpro
 IPR019680; Mediator_Med1_met/fun. 
Pfam
 PF10744; Med1 
SMART
  
PROSITE
  
PRINTS