UniProt Accession

 PTPA_HUMAN; Q15257; A2A347; A9IZU4; B4DXM4; Q15258; Q53GZ3; Q5TZQ2; Q9BUK1; Q9NNZ7; Q9NNZ8; Q9NNZ9 

Genbank Protein ID

 X73478; X86428; X86429; X86430; X86432; X86434; X86435; X86436; X86437; X86438; X86439; X86428; X86429; X86430; X86431; X86432; X86434; X86435; X86436; X86437; X86438; X86439; X86428; X86429; X86432; X86434; X86435; X86436; X86437; X86438; X86439; X86428; X86429; X86430; X86434; X86435; X86436; X86437; X86438; X86439; AK302043; BT020119; AK222788; AL158151; AL158151; CH471090; CH471090; BC002545; BC011605 

Genbank Nucleotide ID

 CAA51873.1; CAA60163.1; CAA60163.1; CAA60163.1; CAA60163.1; CAA60163.1; CAA60163.1; CAA60163.1; CAA60163.1; CAA60163.1; CAA60163.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77601.1; CAB77602.1; CAB77602.1; CAB77602.1; CAB77602.1; CAB77602.1; CAB77602.1; CAB77602.1; CAB77602.1; CAB77602.1; CAB77603.1; CAB77603.1; CAB77603.1; CAB77603.1; CAB77603.1; CAB77603.1; CAB77603.1; CAB77603.1; CAB77603.1; BAG63436.1; AAV38922.1; BAD96508.1; CAM14695.1; CAP58847.2; EAW87876.1; EAW87882.1; AAH02545.1; AAH11605.1 

Protein Name

 Serine/threonine-protein phosphatase 2A activator 

Protein Synonyms/Alias

 PP2A, subunit B', PR53 isoform; Phosphotyrosyl phosphatase activator; PTPA; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B' 

Gene Name

 PPP2R4 

Gene Synonyms/Alias

 PTPA 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
27	TQNFIIPKKEIHTVP	ubiquitination	[1]
62	LNEGVKGKKLTFEYR	acetylation	[2]
63	NEGVKGKKLTFEYRV	ubiquitination	[3]
138	QPSRFGNKAYRTWYA	ubiquitination	[3]
277	PRHFVDEKAVNENHK	ubiquitination	[1]
321	SAVPSWSKVNQGLIR	ubiquitination	[3]
331	QGLIRMYKAECLEKF	acetylation	[2]
331	QGLIRMYKAECLEKF	ubiquitination	[3]
337	YKAECLEKFPVIQHF	acetylation	[2]
337	YKAECLEKFPVIQHF	ubiquitination	[3]
345	FPVIQHFKFGSLLPI	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A. 

Sequence Annotation

 NP_BIND 183 189 ATP.
 NP_BIND 240 242 ATP.
 NP_BIND 342 343 ATP.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Isomerase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Rotamase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 358 AA 

Protein Sequence

Gene Ontology

 GO:0034704; C:calcium channel complex; IDA:BHF-UCL.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0000159; C:protein phosphatase type 2A complex; TAS:HGNC.
 GO:0005524; F:ATP binding; IDA:HGNC.
 GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IBA:RefGenome.
 GO:0046982; F:protein heterodimerization activity; TAS:HGNC.
 GO:0042803; F:protein homodimerization activity; IDA:HGNC.
 GO:0051721; F:protein phosphatase 2A binding; IDA:HGNC.
 GO:0008601; F:protein phosphatase type 2A regulator activity; IDA:HGNC.
 GO:0008160; F:protein tyrosine phosphatase activator activity; IDA:HGNC.
 GO:0006200; P:ATP catabolic process; IDA:GOC.
 GO:0030472; P:mitotic spindle organization in nucleus; IBA:RefGenome.
 GO:0032515; P:negative regulation of phosphoprotein phosphatase activity; IDA:HGNC.
 GO:0035308; P:negative regulation of protein dephosphorylation; IDA:HGNC.
 GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB.
 GO:0032516; P:positive regulation of phosphoprotein phosphatase activity; IDA:HGNC.
 GO:0035307; P:positive regulation of protein dephosphorylation; IDA:HGNC.
 GO:0006457; P:protein folding; IEA:UniProtKB-KW. 

Interpro

 IPR004327; Phstyr_phstse_ac. 

Pfam

 PF03095; PTPA 

SMART

  

PROSITE

  

PRINTS