CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019353
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PRKC apoptosis WT1 regulator protein 
Protein Synonyms/Alias
 Prostate apoptosis response 4 protein; Par-4 
Gene Name
 PAWR 
Gene Synonyms/Alias
 PAR4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
333QENKTLLKVVGQLTRubiquitination[1, 2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down- regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. 
Sequence Annotation
 REGION 145 203 Selective for apoptosis induction in
 REGION 300 340 Leucine-zipper.
 MOTIF 145 161 Nuclear localization signal (By
 MOD_RES 163 163 Phosphothreonine; by PKA (By similarity).  
Keyword
 3D-structure; Apoptosis; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 340 AA 
Protein Sequence
MATGGYRTSS GLGGSTTDFL EEWKAKREKM RAKQNPPGPA PPGGGSSDAA GKPPAGALGT 60
PAAAAANELN NNLPGGAPAA PAVPGPGGVN CAVGSAMLTR AAPGPRRSED EPPAASASAA 120
PPPQRDEEEP DGVPEKGKSS GPSARKGKGQ IEKRKLREKR RSTGVVNIPA AECLDEYEDD 180
EAGQKERKRE DAITQQNTIQ NEAVNLLDPG SSYLLQEPPR TVSGRYKSTT SVSEEDVSSR 240
YSRTDRSGFP RYNRDANVSG TLVSSSTLEK KIEDLEKEVV RERQENLRLV RLMQDKEEMI 300
GKLKEEIDLL NRDLDDIEDE NEQLKQENKT LLKVVGQLTR 340 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0003779; F:actin binding; ISS:UniProtKB.
 GO:0019899; F:enzyme binding; IDA:UniProtKB.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0051017; P:actin filament bundle assembly; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; TAS:ProtInc.
 GO:0006917; P:induction of apoptosis; ISS:UniProtKB.
 GO:0042094; P:interleukin-2 biosynthetic process; IEA:Compara.
 GO:0030889; P:negative regulation of B cell proliferation; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0042130; P:negative regulation of T cell proliferation; IEA:Compara.
 GO:0050860; P:negative regulation of T cell receptor signaling pathway; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0042986; P:positive regulation of amyloid precursor protein biosynthetic process; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR026117; Par-4. 
Pfam
  
SMART
  
PROSITE
  
PRINTS