CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005028
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Catechol O-methyltransferase 
Protein Synonyms/Alias
  
Gene Name
 COMT 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
177QDIIPQLKKKYDVDTubiquitination[1]
178DIIPQLKKKYDVDTLubiquitination[1, 2, 3, 4]
179IIPQLKKKYDVDTLDubiquitination[2, 3, 4]
212EECGLLRKGTVLLADubiquitination[3, 5, 6, 7]
259EVVDGLEKAIYKGPGubiquitination[1, 2, 3, 4, 7]
263GLEKAIYKGPGSEAGubiquitination[1, 3, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. 
Sequence Annotation
 REGION 167 170 S-adenosyl-L-methionine binding.
 METAL 191 191 Magnesium.
 METAL 219 219 Magnesium.
 METAL 220 220 Magnesium.
 BINDING 92 92 S-adenosyl-L-methionine; via amide
 BINDING 122 122 S-adenosyl-L-methionine.
 BINDING 140 140 S-adenosyl-L-methionine.
 BINDING 191 191 S-adenosyl-L-methionine.
 BINDING 194 194 Substrate.
 BINDING 220 220 Substrate.
 BINDING 249 249 Substrate.  
Keyword
 3D-structure; Alternative initiation; Catecholamine metabolism; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Magnesium; Membrane; Metal-binding; Methyltransferase; Neurotransmitter degradation; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 271 AA 
Protein Sequence
MPEAPPLLLA AVLLGLVLLV VLLLLLRHWG WGLCLIGWNE FILQPIHNLL MGDTKEQRIL 60
NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG 120
YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY 180
DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE 240
CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P 271 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0016206; F:catechol O-methyltransferase activity; IEA:EC.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0008171; F:O-methyltransferase activity; TAS:ProtInc.
 GO:0042420; P:dopamine catabolic process; IEA:Compara.
 GO:0008210; P:estrogen metabolic process; IEA:Compara.
 GO:0007565; P:female pregnancy; IEA:Compara.
 GO:0007612; P:learning; IEA:Compara.
 GO:0048609; P:multicellular organismal reproductive process; IEA:Compara.
 GO:0045963; P:negative regulation of dopamine metabolic process; IEA:Compara.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0042136; P:neurotransmitter biosynthetic process; TAS:Reactome.
 GO:0042135; P:neurotransmitter catabolic process; IEA:UniProtKB-KW.
 GO:0050668; P:positive regulation of homocysteine metabolic process; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara.
 GO:0048265; P:response to pain; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 
Interpro
 IPR025782; Catechol_O-MeTrfase.
 IPR017128; Catechol_O-MeTrfase_euk.
 IPR002935; O-MeTrfase_3. 
Pfam
 PF01596; Methyltransf_3 
SMART
  
PROSITE
  
PRINTS