CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037460
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Cyclin-G-associated kinase 
Protein Synonyms/Alias
  
Gene Name
 GAK 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56GQLVEFLKKMESRGPubiquitination[1]
96PIIHRDLKVENLLLSubiquitination[1, 2, 3]
246AARNVNPKSPITELLubiquitination[1, 2, 3]
311ERLFTNLKDTSSKVIubiquitination[1, 3, 4, 5]
316NLKDTSSKVIQSVANubiquitination[5]
326QSVANYAKGDLDISYubiquitination[1]
353EGVESALKNNIEDVRubiquitination[1, 2, 5]
366VRLFLDSKHPGHYAVubiquitination[1]
511TPVPLFSKQRSGCRPubiquitination[1]
538STSQEYDKMRDFKIEubiquitination[1]
603PRNATTVKFAKYDLDubiquitination[1]
1186VAPEQVKKHYRRAVLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1232 AA 
Protein Sequence
MSLLQSALDF LAGPGSLGGA SGRDQSDFVG QTVELGELRL RVRRVLAEGQ LVEFLKKMES 60
RGPLSCDTVL KIFYQTCRAV QHMHRQKPPI IHRDLKVENL LLSNQGTIKL CDFGSATTIS 120
HYPDYSWSAQ RRALVEEEIT RNTTPMYRTP EIIDLYSNFP IGEKQDIWAL GCILYLLCFR 180
QHPFEDGAKL RIVNGKYSIP PHDTQYTVFH SLIRAMLQVN PEERLSIAEV VHQLQEIAAA 240
RNVNPKSPIT ELLEQNGGYG SATLSRGPPP PVGPAGSGYS GGLALAEYDQ PYGGFLDILR 300
GGTERLFTNL KDTSSKVIQS VANYAKGDLD ISYITSRIAV MSFPAEGVES ALKNNIEDVR 360
LFLDSKHPGH YAVYNLSPRT YRPSRFHNRV SECGWAARRA PHLHTLYNIC RNMHAWLRQD 420
HKNVCVVHCM DGRAASAVAV CSFLCFCRLF STAEAAVYMF SMKRCPPGIW PSHKRYIEYM 480
CDMVAEEPIT PHSKPILVRA VVMTPVPLFS KQRSGCRPFC EVYVGDERVA STSQEYDKMR 540
DFKIEDGKAV IPLGVTVQGD VLIVIYHARS TLGGRLQAKM ASMKMFQIQF HTGFVPRNAT 600
TVKFAKYDLD ACDIQEKYPD LFQVNLEVEV EPRDRPSREA PPWENSSMRG LNPKILFSSR 660
EEQQDILSKF GKPELPRQPG STAQYDAGAG SPEAEPTDSD SPPSSSADAS RFLHTLDWQE 720
EKEAETGAEN ASSKESESAL MEDRDESEVS DEGGSPISSE GQEPRADPEP PGLAAGLVQQ 780
DLVFEVETPA VLPEPVPQED GVDLLGLHSE VGAGPAVPPQ ACKAPSSNTD LLSCLLGPPE 840
AASQGPPEDL LSEDPLLLAS PAPPLSVQST PRGGPPAAAD PFGPLLPSSG NNSQPCSNPD 900
LFGEFLNSDS VTVPPSFPSA HSAPPPSCSA DFLHLGDLPG EPSKMTASSS NPDLLGGWAA 960
WTETAASAVA PTPATEGPLF SPGGQPAPCG SQASWTKSQN PDPFADLGDL SSGLQGSPAG 1020
FPPGGFIPKT ATTPKGSSSW QTSRPPAQGA SWPPQAKPPP KACTQPRPNY ASNFSVIGAR 1080
EERGVRAPSF AQKPKVSEND FEDLLSNQGF SSRSDKKGPK TIAEMRKQDL AKDTDPLKLK 1140
LLDWIEGKER NIRALLSTLH TVLWDGESRW TPVGMADLVA PEQVKKHYRR AVLAVHPDKA 1200
AGQPYEQHAK MIFMELNDAW SEFENQGSRP LF 1232 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW. 
Interpro
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR001623; DnaJ_domain.
 IPR011009; Kinase-like_dom.
 IPR014019; Phosphatase_tensin-typ.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR014020; Tensin_phosphatase_C2-dom. 
Pfam
 PF00226; DnaJ
 PF00069; Pkinase
 PF10409; PTEN_C2 
SMART
 SM00271; DnaJ 
PROSITE
 PS51182; C2_TENSIN
 PS50076; DNAJ_2
 PS51181; PPASE_TENSIN
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS