CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011390
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromatin modification-related protein EAF1 
Protein Synonyms/Alias
 ESA1-associated factor 1; Vacuolar import and degradation protein 21 
Gene Name
 EAF1 
Gene Synonyms/Alias
 VID21; YDR359C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
280MNDYIPSKIPQALPLacetylation[1]
848SPIPSNGKSSSNLARacetylation[1, 2]
Reference
 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. 
Sequence Annotation
 DOMAIN 346 425 HSA.
 DOMAIN 642 704 Myb-like.
 MOD_RES 841 841 Phosphoserine.
 MOD_RES 971 971 Phosphothreonine.  
Keyword
 Activator; Chromatin regulator; Complete proteome; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 982 AA 
Protein Sequence
MSSRPSSAVP NSASLSEDQS SDRSKFPKAD DLIDERDRKL TELYCVSRLN QLLELTDENK 60
LRKEIDAFLK KNDIRRGIRF DEASLPKLLH TAATPITKKK LKDVNLINVP NQRLSDSKMS 120
RELPENSENV SVKSESHFVP SHDNSIRENM MDSLRPAEKT GGMWNKRPLE STMGGEEERH 180
EKRQKMQSQS LESSNNSEMA SLPISPRPPV PNALAHYTYY ENIEYPPADP TEVQPAVKFK 240
DPLIKNIMAK EIDTSDHYNE NNVDALETVF LLMNDYIPSK IPQALPLAEL KYMSQTLPLI 300
NLIPRAHKAL TTNIINNALN EARITVVGSR IEELRRLGLW SLRQPKRFID PWKQHNTHQN 360
ILLEEAKWMQ ADFKEGHKYK VAICTAMAQA IKDYWTYGEI CCVKRKTLLP GKENKLSDDG 420
RISEKSGRPS DTSRNDSDIS IAGKDDIGII ANVDDITEKE SAAANDNDEN GKNEAGAKSD 480
FDFADGLLSQ EGAHDQIISS IDTKLLLKKP SSSSEVVLIQ HEVAASSALI ETEESKKELA 540
PPFKLSIFVD ELNTFEKTLI QDLPLYNGIN EERPKKDDSL PFIPISKSVV SLDDNGFYKL 600
LERQLIDEEP SISQLSKRRG MFYGNRRNHY LRPPAVPSLR YLQNRTPTIW LSEDDQELVK 660
NINTYGYNWE LISAHMTHRL TYSYLSNIER RTPWQCFERF VQLNERFNFS DLKGPRAHSA 720
QQWLIEAHKF QQRQNRRISP LGVNTESIQR GHRRLRWASM FEAIRKCMKK RENTPRPNPT 780
QPRKPLDCKN MKVPTPAEMS LLKAQRDEAL RRDIQLRRTV KNRLQQRQQQ SQQAHSSRAQ 840
SPIPSNGKSS SNLARNGQAS APRPNQKQYT EQDIIESYSR KLLEQKPDIG PEMALKAAKN 900
YYRTLREQQQ QLKQHQIQQQ RQQLQEESSH VQQLQQLQPG SQAPPPKSSP SQSSLSNISN 960
INSAPRIKSP TPQEILQRFQ KQ 982 
Gene Ontology
 GO:0035267; C:NuA4 histone acetyltransferase complex; IPI:SGD.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0043623; P:cellular protein complex assembly; IMP:SGD.
 GO:0016568; P:chromatin modification; IPI:SGD.
 GO:0006281; P:DNA repair; IDA:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR013999; HAS_subgr.
 IPR014012; Helicase/SANT-assoc_DNA-bd.
 IPR009057; Homeodomain-like.
 IPR006562; HSA.
 IPR017877; Myb-like_dom.
 IPR001005; SANT/Myb. 
Pfam
 PF07529; HSA 
SMART
 SM00573; HSA
 SM00717; SANT 
PROSITE
 PS51204; HSA
 PS50090; MYB_LIKE 
PRINTS