CPLM-012019

Genbank Nucleotide ID

Heterogeneous nuclear ribonucleoprotein A0

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
8	MENSQLCKLFIGGLN	ubiquitination	[1, 2]
45	VVVNPQTKRSRCFGF	ubiquitination	[1, 2, 3]
80	DGNTVELKRAVSRED	ubiquitination	[1, 2, 4, 5]
96	ARPGAHAKVKKLFVG	acetylation	[6]
99	GAHAKVKKLFVGGLK	ubiquitination	[2, 6, 7, 8]
106	KLFVGGLKGDVAEGD	ubiquitination	[1, 2, 3]
126	SQFGTVEKAEIIADK	ubiquitination	[2]
133	KAEIIADKQSGKKRG	acetylation	[6]
133	KAEIIADKQSGKKRG	ubiquitination	[1, 2, 3, 5, 6]
137	IADKQSGKKRGFGFV	ubiquitination	[1]
154	QNHDAADKAAVVKFH	ubiquitination	[1, 2, 6]
159	ADKAAVVKFHPIQGH	ubiquitination	[2, 6]
172	GHRVEVKKAVPKEDI	ubiquitination	[1, 2]
176	EVKKAVPKEDIYSGG	ubiquitination	[2, 6]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

mRNA-binding component of ribonucleosomes. Specifically binds AU-rich element (ARE)-containing mRNAs. Involved in post- transcriptional regulation of cytokines mRNAs.

DOMAIN 7 86 RRM 1.
DOMAIN 98 175 RRM 2.
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 84 84 Phosphoserine; by MAPKAPK2.
MOD_RES 188 188 Phosphoserine.
MOD_RES 291 291 Dimethylated arginine.

Acetylation; Complete proteome; Direct protein sequencing; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
GO:0017091; F:AU-rich element binding; IDA:UniProtKB.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0070935; P:3'-UTR-mediated mRNA stabilization; ISS:UniProtKB.
GO:0006954; P:inflammatory response; ISS:UniProtKB.
GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
GO:0032496; P:response to lipopolysaccharide; ISS:UniProtKB.

IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.