UniProt Accession

 HAS1_YEAST; Q03532; D6W0B7 

Genbank Protein ID

 X80836; BK006946 

Genbank Nucleotide ID

 CAA56799.1; DAA10191.1 

Protein Name

 ATP-dependent RNA helicase HAS1 

Protein Synonyms/Alias

 Helicase associated with SET1 protein 1 

Gene Name

 HAS1 

Gene Synonyms/Alias

 YMR290C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
43	EQTTCVEKFEELKLS	ubiquitination	[1]
48	VEKFEELKLSQPTLK	acetylation	[2]
55	KLSQPTLKAIEKMGF	ubiquitination	[1]
87	RDVLGAAKTGSGKTL	ubiquitination	[1]
431	NVQSQLEKLIKSNYY	acetylation	[2]
444	YYLHQTAKDGYRSYL	acetylation	[2]
444	YYLHQTAKDGYRSYL	methylation	[3]
467	KTVYQIDKLDLAKVA	acetylation	[2]
494	ITIGASGKTPNTKRR	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074] 

Functional Description

 ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. 

Sequence Annotation

 DOMAIN 73 249 Helicase ATP-binding.
 DOMAIN 263 433 Helicase C-terminal.
 NP_BIND 86 93 ATP.
 MOTIF 42 70 Q motif.
 MOTIF 196 199 DEAD box.
 MOTIF 275 291 Bipartite nuclear localization signal
 MOD_RES 12 12 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 505 AA 

Protein Sequence

Gene Ontology

 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0030687; C:preribosome, large subunit precursor; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IDA:SGD.
 GO:0003723; F:RNA binding; IDA:SGD.
 GO:0006364; P:rRNA processing; IMP:SGD. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR025313; DUF4217.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF13959; DUF4217
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS