UniProt Accession

 PURU_ECOLI; P37051 

Genbank Protein ID

 L20251; M64675; U00096; AP009048 

Genbank Nucleotide ID

 AAC36846.1; AAA16860.1; AAC74314.1; BAA36100.1 

Protein Name

 Formyltetrahydrofolate deformylase 

Protein Synonyms/Alias

 Formyl-FH(4) hydrolase 

Gene Name

 purU 

Gene Synonyms/Alias

 tgs; ychI; b1232; JW1220 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
17	RTICPDQKGLIARIT	acetylation	[1]
29	RITNICYKHELNIVQ	acetylation	[1]
151	TRNEHDQKMADAIDA	acetylation	[1]
168	PDYVVLAKYMRVLTP	acetylation	[1]
184	FVARFPNKIINIHHS	acetylation	[1]
255	RAGRDVEKNVLSRAL	acetylation	[1, 2]
264	VLSRALYKVLAQRVF	acetylation	[1, 3]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

 Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the PurT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool. 

Sequence Annotation

 DOMAIN 8 86 ACT.
 ACT_SITE 225 225 By similarity.  

Keyword

 Complete proteome; Direct protein sequencing; Hydrolase; One-carbon metabolism; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 280 AA 

Protein Sequence

Gene Ontology

 GO:0016597; F:amino acid binding; IEA:InterPro.
 GO:0008864; F:formyltetrahydrofolate deformylase activity; IDA:EcoCyc.
 GO:0016742; F:hydroxymethyl-, formyl- and related transferase activity; IEA:InterPro.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009257; P:10-formyltetrahydrofolate biosynthetic process; TAS:EcoCyc.
 GO:0006730; P:one-carbon metabolic process; TAS:EcoCyc.
 GO:0009152; P:purine ribonucleotide biosynthetic process; IDA:EcoCyc. 

Interpro

 IPR002912; ACT_dom.
 IPR002376; Formyl_transf_N.
 IPR004810; PurU. 

Pfam

 PF01842; ACT
 PF00551; Formyl_trans_N 

SMART

  

PROSITE

 PS51671; ACT 

PRINTS

 PR01575; FFH4HYDRLASE.