CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005002
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zona pellucida sperm-binding protein 3 
Protein Synonyms/Alias
 Sperm receptor; ZP3A/ZP3B; Zona pellucida glycoprotein 3; Zp-3; Zona pellucida protein C; Processed zona pellucida sperm-binding protein 3 
Gene Name
 ZP3 
Gene Synonyms/Alias
 ZP3A; ZP3B; ZPC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
64KDLFGTGKLIRAADLubiquitination[1]
185EENWNAEKRSPTFHLubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. 
Sequence Annotation
 DOMAIN 45 307 ZP.
 MOD_RES 23 23 Pyrrolidone carboxylic acid.
 CARBOHYD 125 125 N-linked (GlcNAc...).
 CARBOHYD 147 147 N-linked (GlcNAc...).
 CARBOHYD 156 156 O-linked (GalNAc...) (By similarity).
 CARBOHYD 162 162 O-linked (GalNAc...) (By similarity).
 CARBOHYD 163 163 O-linked (GalNAc...) (By similarity).
 CARBOHYD 226 226 N-linked (GlcNAc...) (Potential).
 CARBOHYD 272 272 N-linked (GlcNAc...).
 DISULFID 46 140
 DISULFID 78 99
 DISULFID 217 282
 DISULFID 239 300  
Keyword
 Alternative splicing; Cell membrane; Cleavage on pair of basic residues; Complete proteome; Disulfide bond; Extracellular matrix; Fertilization; Glycoprotein; Membrane; Polymorphism; Pyrrolidone carboxylic acid; Receptor; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 424 AA 
Protein Sequence
MELSYRLFIC LLLWGSTELC YPQPLWLLQG GASHPETSVQ PVLVECQEAT LMVMVSKDLF 60
GTGKLIRAAD LTLGPEACEP LVSMDTEDVV RFEVGLHECG NSMQVTDDAL VYSTFLLHDP 120
RPVGNLSIVR TNRAEIPIEC RYPRQGNVSS QAILPTWLPF RTTVFSEEKL TFSLRLMEEN 180
WNAEKRSPTF HLGDAAHLQA EIHTGSHVPL RLFVDHCVAT PTPDQNASPY HTIVDFHGCL 240
VDGLTDASSA FKVPRPGPDT LQFTVDVFHF ANDSRNMIYI TCHLKVTLAE QDPDELNKAC 300
SFSKPSNSWF PVEGSADICQ CCNKGDCGTP SHSRRQPHVM SQWSRSASRN RRHVTEEADV 360
TVGPLIFLDR RGDHEVEQWA LPSDTSVVLL GVGLAVVVSL TLTAVILVLT RRCRTASHPV 420
SASE 424 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0031012; C:extracellular matrix; IDA:UniProtKB.
 GO:0005615; C:extracellular space; IMP:UniProtKB.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005771; C:multivesicular body; ISS:UniProtKB.
 GO:0002081; C:outer acrosomal membrane; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0005578; C:proteinaceous extracellular matrix; IEA:UniProtKB-SubCell.
 GO:0030246; F:carbohydrate binding; IDA:UniProtKB.
 GO:0005384; F:manganese ion transmembrane transporter activity; ISS:UniProtKB.
 GO:0004871; F:signal transducer activity; IMP:UniProtKB.
 GO:0015279; F:store-operated calcium channel activity; IDA:UniProtKB.
 GO:0007339; P:binding of sperm to zona pellucida; IDA:UniProtKB.
 GO:0001825; P:blastocyst formation; ISS:UniProtKB.
 GO:0035803; P:egg coat formation; ISS:UniProtKB.
 GO:0002455; P:humoral immune response mediated by circulating immunoglobulin; ISS:UniProtKB.
 GO:0006886; P:intracellular protein transport; ISS:UniProtKB.
 GO:2000360; P:negative regulation of binding of sperm to zona pellucida; IDA:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0048599; P:oocyte development; ISS:UniProtKB.
 GO:0048015; P:phosphatidylinositol-mediated signaling; ISS:UniProtKB.
 GO:2000368; P:positive regulation of acrosomal vesicle exocytosis; IDA:UniProtKB.
 GO:2000344; P:positive regulation of acrosome reaction; IDA:UniProtKB.
 GO:2000388; P:positive regulation of antral ovarian follicle growth; ISS:UniProtKB.
 GO:0090280; P:positive regulation of calcium ion import; IDA:UniProtKB.
 GO:0002922; P:positive regulation of humoral immune response; IDA:UniProtKB.
 GO:0032729; P:positive regulation of interferon-gamma production; ISS:UniProtKB.
 GO:0032753; P:positive regulation of interleukin-4 production; ISS:UniProtKB.
 GO:0002687; P:positive regulation of leukocyte migration; ISS:UniProtKB.
 GO:2000386; P:positive regulation of ovarian follicle development; ISS:UniProtKB.
 GO:0010513; P:positive regulation of phosphatidylinositol biosynthetic process; ISS:UniProtKB.
 GO:0045860; P:positive regulation of protein kinase activity; IDA:UniProtKB.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; ISS:UniProtKB.
 GO:0042102; P:positive regulation of T cell proliferation; ISS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0001809; P:positive regulation of type IV hypersensitivity; ISS:UniProtKB.
 GO:0070528; P:protein kinase C signaling cascade; IMP:UniProtKB. 
Interpro
 IPR001507; ZP_dom.
 IPR017977; ZP_dom_CS. 
Pfam
 PF00100; Zona_pellucida 
SMART
 SM00241; ZP 
PROSITE
 PS00682; ZP_1
 PS51034; ZP_2 
PRINTS
 PR00023; ZPELLUCIDA.