UniProt Accession

 LRWD1_HUMAN; Q9UFC0; A8K4K2; B2R9G2; Q8N0T9; Q8WV43; Q96GJ2 

Genbank Protein ID

 AK290967; AK313771; AL133057; AC093668; CH471197; BC009436; BC018769; BC030547 

Genbank Nucleotide ID

 BAF83656.1; BAG36509.1; CAB61382.2; EAW50250.1; AAH09436.1; AAH18769.1; AAH30547.1 

Protein Name

 Leucine-rich repeat and WD repeat-containing protein 1 

Protein Synonyms/Alias

 ORC-associated protein; ORCA; Origin recognition complex-associated protein 

Gene Name

 LRWD1 

Gene Synonyms/Alias

 ORCA 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
41	LSEHLDPKLLCRLTQ	ubiquitination	[1]
121	FLLPTLRKVNGKDAS	ubiquitination	[1]
151	RVTAHWEKFMATLGP	ubiquitination	[1]
171	KAQADFVKSAVRDVR	ubiquitination	[1, 2]
246	PLSLSPSKRACASPS	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recognizes and binds these repressive marks by itself or needs additional factors. 

Sequence Annotation

 REPEAT 22 43 LRR 1.
 REPEAT 48 69 LRR 2.
 REPEAT 70 91 LRR 3.
 REPEAT 92 113 LRR 4.
 REPEAT 390 430 WD 1.
 REPEAT 441 480 WD 2.
 REPEAT 495 534 WD 3.
 REPEAT 551 590 WD 4.
 REPEAT 615 647 WD 5.
 MOD_RES 212 212 Phosphoserine.
 MOD_RES 243 243 Phosphoserine.
 MOD_RES 251 251 Phosphoserine.
 MOD_RES 259 259 Phosphoserine.  

Keyword

 Centromere; Chromatin regulator; Chromosome; Complete proteome; DNA replication; Leucine-rich repeat; Nucleus; Phosphoprotein; Reference proteome; Repeat; Telomere; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 647 AA 

Protein Sequence

Gene Ontology

 GO:0005721; C:centromeric heterochromatin; IDA:UniProtKB.
 GO:0005664; C:nuclear origin of replication recognition complex; IDA:UniProtKB.
 GO:0031933; C:telomeric heterochromatin; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0008327; F:methyl-CpG binding; IDA:UniProtKB.
 GO:0035064; F:methylated histone residue binding; IDA:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0006325; P:chromatin organization; IMP:UniProtKB.
 GO:0006270; P:DNA replication initiation; TAS:UniProtKB.
 GO:0071169; P:establishment of protein localization to chromatin; IDA:UniProtKB.
 GO:0000080; P:G1 phase of mitotic cell cycle; IMP:UniProtKB. 

Interpro

 IPR001611; Leu-rich_rpt.
 IPR003591; Leu-rich_rpt_typical-subtyp.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00369; LRR_TYP
 SM00320; WD40 

PROSITE

 PS51450; LRR
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS