CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012461
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polycomb protein SUZ12 
Protein Synonyms/Alias
 Chromatin precipitated E2F target 9 protein; ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog 
Gene Name
 SUZ12 
Gene Synonyms/Alias
 CHET9; JJAZ1; KIAA0160 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
72GAAVLPVKKPKMEHVsumoylation[1]
75VLPVKKPKMEHVQADsumoylation[1]
92LFLQAFEKPTQIYRFubiquitination[2]
341WETILDGKRLPPFETubiquitination[2, 3, 4, 5]
368WTGETNDKSTAPIAKubiquitination[2, 3, 5]
375KSTAPIAKPLATRNSubiquitination[3, 5]
537VCRPKRTKASMSEFLubiquitination[4]
595DPEWLREKTITQIEEubiquitination[4]
Reference
 [1] The polycomb repressive complex 2 is a potential target of SUMO modifications.
 Riising EM, Boggio R, Chiocca S, Helin K, Pasini D.
 PLoS One. 2008 Jul 16;3(7):e2704. [PMID: 18628979]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. 
Sequence Annotation
 ZN_FING 448 471 C2H2-type.
 REGION 563 639 VEFS-box.
 MOD_RES 546 546 Phosphoserine.
 MOD_RES 583 583 Phosphoserine.
 CROSSLNK 72 72 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 73 73 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 75 75 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Chromatin regulator; Chromosomal rearrangement; Complete proteome; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 739 AA 
Protein Sequence
MAPQKHGGGG GGGSGPSAGS GGGGFGGSAA VAAATASGGK SGGGSCGGGG SYSASSSSSA 60
AAAAGAAVLP VKKPKMEHVQ ADHELFLQAF EKPTQIYRFL RTRNLIAPIF LHRTLTYMSH 120
RNSRTNIKRK TFKVDDMLSK VEKMKGEQES HSLSAHLQLT FTGFFHKNDK PSPNSENEQN 180
SVTLEVLLVK VCHKKRKDVS CPIRQVPTGK KQVPLNPDLN QTKPGNFPSL AVSSNEFEPS 240
NSHMVKSYSL LFRVTRPGRR EFNGMINGET NENIDVNEEL PARRKRNRED GEKTFVAQMT 300
VFDKNRRLQL LDGEYEVAMQ EMEECPISKK RATWETILDG KRLPPFETFS QGPTLQFTLR 360
WTGETNDKST APIAKPLATR NSESLHQENK PGSVKPTQTI AVKESLTTDL QTRKEKDTPN 420
ENRQKLRIFY QFLYNNNTRQ QTEARDDLHC PWCTLNCRKL YSLLKHLKLC HSRFIFNYVY 480
HPKGARIDVS INECYDGSYA GNPQDIHRQP GFAFSRNGPV KRTPITHILV CRPKRTKASM 540
SEFLESEDGE VEQQRTYSSG HNRLYFHSDT CLPLRPQEME VDSEDEKDPE WLREKTITQI 600
EEFSDVNEGE KEVMKLWNLH VMKHGFIADN QMNHACMLFV ENYGQKIIKK NLCRNFMLHL 660
VSMHDFNLIS IMSIDKAVTK LREMQQKLEK GESASPANEE ITEEQNGTAN GFSEINSKEK 720
ALETDSVSGV SKQSKKQKL 739 
Gene Ontology
 GO:0035098; C:ESC/E(Z) complex; IDA:UniProtKB.
 GO:0001739; C:sex chromatin; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0042054; F:histone methyltransferase activity; IMP:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0035064; F:methylated histone residue binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0016574; P:histone ubiquitination; IEA:Compara.
 GO:0045596; P:negative regulation of cell differentiation; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR019135; Polycomb_protein_VEFS-Box.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF09733; VEFS-Box 
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS