UniProt Accession

 KNG1_MOUSE; O08677; O08676; Q32MX7; Q6S9I1; Q91XK5 

Genbank Protein ID

 D84415; D84435; AY462058; AY660571; AK005547; BC018158; BC108936 

Genbank Nucleotide ID

 BAA19742.1; BAA19743.1; AAR88632.1; AAT70087.1; AAH18158.1; AAI08937.1 

Protein Name

 Kininogen-1 

Protein Synonyms/Alias

 Kininogen-1 heavy chain; Bradykinin; Kininogen-1 light chain 

Gene Name

 Kng1 

Gene Synonyms/Alias

 Kng 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
64	HRVIEGTKTDGSPTF	ubiquitination	[1]
75	SPTFYSFKYLIKEGN	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting (By similarity). 

Sequence Annotation

 DOMAIN 28 131 Cystatin kininogen-type 1.
 DOMAIN 150 253 Cystatin kininogen-type 2.
 DOMAIN 272 375 Cystatin kininogen-type 3.
 MOD_RES 331 331 Phosphoserine (By similarity).
 CARBOHYD 82 82 N-linked (GlcNAc...).
 CARBOHYD 168 168 N-linked (GlcNAc...).
 CARBOHYD 204 204 N-linked (GlcNAc...).
 CARBOHYD 242 242 N-linked (GlcNAc...).
 DISULFID 28 631 Interchain (between heavy and light
 DISULFID 83 94 By similarity.
 DISULFID 107 125 By similarity.
 DISULFID 141 144 By similarity.
 DISULFID 205 217 By similarity.
 DISULFID 228 247 By similarity.
 DISULFID 263 266 By similarity.
 DISULFID 327 339 By similarity.
 DISULFID 350 369 By similarity.  

Keyword

 Alternative splicing; Blood coagulation; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Hemostasis; Inflammatory response; Phosphoprotein; Protease inhibitor; Reference proteome; Repeat; Secreted; Signal; Thiol protease inhibitor; Vasoactive; Vasodilator. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 661 AA 

Protein Sequence

Gene Ontology

 GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
 GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
 GO:0007596; P:blood coagulation; IEA:UniProtKB-KW.
 GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
 GO:0042311; P:vasodilation; IEA:UniProtKB-KW. 

Interpro

 IPR002395; Kininogen.
 IPR027358; Kininogen-type_cystatin_dom.
 IPR000010; Prot_inh_cystat.
 IPR018073; Prot_inh_cystat_CS. 

Pfam

 PF00031; Cystatin 

SMART

 SM00043; CY 

PROSITE

 PS00287; CYSTATIN
 PS51647; CYSTATIN_KININOGEN 

PRINTS

 PR00334; KININOGEN.