UniProt Accession

 ISCR_ECOLI; P0AGK8; P77484 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC75584.1; BAA16425.1 

Protein Name

 HTH-type transcriptional regulator IscR 

Protein Synonyms/Alias

  

Gene Name

 iscR 

Gene Synonyms/Alias

 yfhP; b2531; JW2515 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
103	GGCQGGDKCLTHALW	acetylation	[1]
159	TQDAIDVKLRA****	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA. 

Sequence Annotation

 DOMAIN 2 131 HTH rrf2-type.
 DNA_BIND 28 51 H-T-H motif (By similarity).
 METAL 92 92 Iron-sulfur (2Fe-2S) (Potential).
 METAL 98 98 Iron-sulfur (2Fe-2S) (Potential).
 METAL 104 104 Iron-sulfur (2Fe-2S) (Potential).  

Keyword

 2Fe-2S; 3D-structure; Activator; Complete proteome; DNA-binding; Iron; Iron-sulfur; Metal-binding; Reference proteome; Repressor; Stress response; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 162 AA 

Protein Sequence

Gene Ontology

 GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0003690; F:double-stranded DNA binding; IEA:HAMAP.
 GO:0005506; F:iron ion binding; IEA:HAMAP.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:HAMAP.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR010242; TF_HTH_IscR.
 IPR000944; Tscrpt_reg_Rrf2-like.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF02082; Rrf2 

SMART

  

PROSITE

 PS01332; HTH_RRF2_1
 PS51197; HTH_RRF2_2 

PRINTS